Coexpression within Integrated Mitochondrial Pathways Reveals Different Networks in Normal and Chemically Treated Transcriptomes.
Coexpression within Integrated Mitochondrial Pathways Reveals Different Networks in Normal and Chemically Treated Transcriptomes.
复制标题
DOI：
10.1155/2014/452891
复制
发表时间：
2014
影响因子：
2.9
通讯作者：
Liu J
中科院分区：
文献类型：
--
作者：
Chen C;Hyun TK;Han X;Feng Z;Li Y;Liu X;Liu J
作为能量生产者，线粒体在多种细胞过程中发挥着关键作用。虽然一些证据表明，线粒体呼吸复合物（MRCs）的差异表达对线粒体功能有显着的影响，在整个共表达网络中的综合MRCs的作用尚未被揭示。在这项研究中，我们构建了基于不同组织和化学处理的微阵列数据集的共表达网络，以探讨整合的MRCs在共表达网络中的作用以及不同化学物质对线粒体网络的影响。通过将MRC分组为一个种子靶标，超几何分布使我们能够鉴定与整个MRC显著共表达的基因。在正常组织转录组数据集中，46个MRC基因（约占测试MRC基因的78%）之间的共表达是显著的。这些MRC基因与涉及“肌肉系统过程”、“代谢过程”和“神经退行性疾病途径”的基因共表达，而在化学处理的组织中，这些基因的共表达大多消失。这些结果表明，化学刺激改变MRC基因的正常共表达网络。总之，从不同的共表达网络获得的数据集是关于线粒体生物发生的信息，应该有助于理解药物对线粒体功能的副作用。
As energy producers, mitochondria play a pivotal role in multiple cellular processes. Although several lines of evidence suggest that differential expression of mitochondrial respiratory complexes (MRCs) has a significant impact on mitochondrial function, the role of integrated MRCs in the whole coexpression network has yet to be revealed. In this study, we construct coexpression networks based on microarray datasets from different tissues and chemical treatments to explore the role of integrated MRCs in the coexpression network and the effects of different chemicals on the mitochondrial network. By grouping MRCs as one seed target, the hypergeometric distribution allowed us to identify genes that are significantly coexpress with whole MRCs. Coexpression among 46 MRC genes (approximately 78% of MRC genes tested) was significant in the normal tissue transcriptome dataset. These MRC genes are coexpressed with genes involved in the categories “muscle system process,” “metabolic process,” and “neurodegenerative disease pathways,” whereas, in the chemically treated tissues, coexpression of these genes mostly disappeared. These results indicate that chemical stimuli alter the normal coexpression network of MRC genes. Taken together, the datasets obtained from the different coexpression networks are informative about mitochondrial biogenesis and should contribute to understanding the side effects of drugs on mitochondrial function.
登录
查看更多内容
影响因子：
7
作者：
Lee, HK;Hsu, AK;Pavlidis, P
通讯作者：
Pavlidis, P
影响因子：
3.7
作者：
Hudson NJ;Reverter A;Wang Y;Greenwood PL;Dalrymple BP
通讯作者：
Dalrymple BP
DOI：
10.1073/pnas.1107819108
发表时间：
2011-09-13
影响因子：
11.1
作者：
Dudkina, Natalya V.;Kudryashev, Mikhail;Boekema, Egbert J.
通讯作者：
Boekema, Egbert J.
影响因子：
--
作者：
Lehtinen, Sonja;Marsellach, Francesc Xavier;Pancaldi, Vera
通讯作者：
Pancaldi, Vera
影响因子：
4.8
作者：
Hunt, MC;Solaas, K;Alexson, SEH
通讯作者：
Alexson, SEH