Chromosome changes (trisomies #15 and 17) associated with tumor progression in leukemias induced by radiation leukemia virus.
Chromosome changes (trisomies #15 and 17) associated with tumor progression in leukemias induced by radiation leukemia virus.
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染色体变化（三体性
DOI：
10.1093/jnci/61.1.227
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发表时间：
1978
期刊：
影响因子：
--
通讯作者：
G. Klein
中科院分区：
文献类型：
--
作者：
F. Wiener;S. Ohno;J. Spira;N. Haran‐Ghera;G. Klein
一个实验系统的开发，允许非随机的染色体变化，发生在放射性白血病病毒（RadLV）诱导的淋巴瘤，以跟踪肿瘤进展。将来自雌性近交系C57 BL/6小鼠的RadLV变体诱导的白血病前期和白血病细胞注射到相同品系的雄性动物中。由于所有供体均为女性，所有受体均为男性，因此使用性染色体补体（XX和XY）将白血病前细胞和白血病细胞与宿主来源的细胞区分开来。G显带分析显示，超过50%的接种了白血病前细胞并发展为白血病的动物具有41条染色体的肿瘤干细胞系，其中染色体#15有三裂。在接种了明显的白血病细胞并发生肿瘤进展的动物中，G-显带是染色体#17的额外三体。细胞遗传学数据有力地表明，染色体#15的三体性是在RadLV诱导的白血病前细胞转化为完全自主的肿瘤细胞的过程中发生的第一个特异性肿瘤相关染色体变化。
An experimental system was developed that permitted nonrandom chromosome changes that occur in radiation leukemia virus (RadLV)-induced lymphomas to be followed during tumor progression. RadLV variant-induced preleukemia and leukemia cells originating from female inbred C57BL/6 mice were injected into male animals of the same strain. Since all donors were females and all recipients were males, the sex chromosome complements (XX and XY) were used to distinguish the preleukemia and leukemia cells from those of host origin. The G-banding analysis revealed that more than 50% of animals that were inoculated with preleukemia cells and that developed leukemia possessed tumor stem-lines of 41 chromosomes with a tristomy of chromosome #15. In animals inoculated with overt leukemia cells and in which tumor progression occurred, the G-banding an additional trisomy of chromosome #17. The cytogenic data strongly suggested that the trisomy of chromosome #15 was the first specific tumor-associated chromosome change that occurred in the process of conversion of RadLV-induced preleukemia cells to fully autonomous tumor cells.