NEURITE EXTENSION ACROSS REGIONS OF LOW CELL-SUBSTRATUM ADHESIVITY - IMPLICATIONS FOR THE GUIDEPOST HYPOTHESIS OF AXONAL PATHFINDING
NEURITE EXTENSION ACROSS REGIONS OF LOW CELL-SUBSTRATUM ADHESIVITY - IMPLICATIONS FOR THE GUIDEPOST HYPOTHESIS OF AXONAL PATHFINDING
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DOI：
10.1016/0012-1606(86)90334-9
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发表时间：
1986-10-01
影响因子：
2.7
通讯作者：
LETOURNEAU, PC
中科院分区：
文献类型：
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作者：
HAMMARBACK, JA;LETOURNEAU, PC
根据粘着“路标”假说，先驱轴突遵循由特定的非相邻细胞（路标细胞）标记的路径。这一假设意味着轴突和路标细胞之间的高粘附性有利于轴突穿过低粘附性组织或路标细胞之间的空间延伸。本研究探讨了高粘附性基质在体外促进轴突延伸穿过低粘附性基质的能力。将分离的鸡胚背根神经节神经元培养在由高粘附性基质结合层粘连蛋白区域组成的基质上（即，模型粘合剂路标）由低粘性琼脂糖基质分开。增加这些路标的细胞-基质粘附性导致跨越低粘附性基质的给定宽度的神经突的百分比增加。神经突尖端的丝柄突可以延伸到低粘附性基底上。显然，丝状伪足与高粘附性路标的接触使神经突能够延伸穿过介入的低粘附性基质。一些神经突在体外跨越的低粘附性基底层不连续的最大宽度与昆虫中一些假定的路标细胞之间的距离相当。与粘合剂路标假说一致，这些研究结果表明，神经突延伸的不连续的细胞基质的粘附性。
According to the adhesive "guidepost" hypothesis, pioneer axons follow pathways marked by specific nonadjacent cells (guidepost cells). The hypothesis implies that high adhesivity between extending axons and guidepost cells facilitates axon extension across low-adhesivity tissues or spaces between guidepost cells. This study investigates the ability of a high-adhesivity substratum to promote axonal extension across a low-adhesivity substratum in vitro. Dissociated chick embryo dorsal root ganglion neurons are cultured on a substratum consisting of areas of high-adhesivity substratum-bound laminin (i.e., model adhesive guideposts) separated by a low-adhesivity agarose substratum. Increasing the cell-substratum adhesivity of these guideposts results in an increase in the percentage of neurites spanning a given width of the low-adhesivity substratum. Filopodial processes at the tips of neurites can extend over the low-adhesivity substratum. Apparently, filopodial contact with high-adhesivity guideposts enables neurites to extend across intervening low-adhesivity substrata. The maximum width of low-adhesivity substratum discontinuities spanned by some neurites in vitro is comparable to the distance between some putative guidepost cells in insects. Consistent with the adhesive guidepost hypothesis, these findings demonstrate neurite extension on a substratum of discontinuous cell-substratum adhesivity.