Aromatic L-amino acid decarboxylase in the rat brain: immunocytochemical localization during prenatal development.
Aromatic L-amino acid decarboxylase in the rat brain: immunocytochemical localization during prenatal development.
复制标题
大鼠脑中的芳香族 L-氨基酸脱羧酶：产前发育期间的免疫细胞化学定位。
DOI：
10.1016/0306-4522(86)90183-1
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发表时间：
1986
期刊：
影响因子：
3.3
通讯作者：
Jaeger,CB
中科院分区：
文献类型：
--
作者：
Jaeger,CB
含有芳香族氨基酸脱羧酶的免疫细胞化学标记的细胞位于胎龄E15-E19的大鼠胚胎的脑中。含有芳香族氨基酸脱羧酶但缺乏酪氨酸羟化酶或吲哚胺5-羟色胺的细胞组被称为“D”组。应用解剖标志、细胞构筑和乙酰胆碱酯酶组织化学染色等方法确定D组的位置。在E15胚胎中，存在三个“D”组。第一个出现的，命名为D1，位于脊髓中，之前已经被证明。在下丘脑沟深处的中央前脑壁上发现一个大的“D”细胞簇。这一组分布在背侧丘脑腹侧背侧区和腹侧背侧下丘脑。最喙的“D”组，D14，发生在腹侧端脑内侧的黑质纹状体投射的纤维。D14是早期组中最小的。在E16和E17胚胎背二和中脑“D”组首先检测到。在个体发育的过程中，免疫反应细胞的大量增加发生和隔离的大型中央前脑集群成几个rostrally和横向分布的“D”组发生。一些“D”组，发生在成年人的大脑中不存在于E19 embryon.This研究提供了几个独特的细胞群在大鼠胚胎的大脑中的定位和它们的外观在不同的妊娠阶段的第一份报告。这也进一步支持了芳香族氨基酸脱羧酶染色强度的变化可能是不同单胺神经元的特征的观点。
Immunocytochemically labeled cells containing the enzyme aromaticl-amino acid decar☐ylase were localized in the brain of rat embryos at gestational age E15–E19. Cell groups that contained aromaticl-amino acid decar☐ylase but lacked either the enzyme tyrosine hydroxylase or the indolamine serotonin were referred to as “D” groups. Anatomical landmarks, cytoarchitectonic structure and histochemical staining for acetylcholinesterase were used to delineate the position of “D” groups. In the E15 embryo three “D” groups existed. The first to appear, named D1, was located in the spinal cord and had been demonstrated before. A large “D” cell cluster was found in the walls of the central forebrain deep to the hypothalamic sulcus. This group distributed dorsally in the ventral dorsal thalamic region and ventrally in the dorsal hypothalamus. The rostral-most “D” group, D14, occurred in the ventral telencephalon just medial to fibers of the nigrostriatal projection. D14 was the smallest of the early groups. In E16 and E17 embryos dorsal di- and mesencephalic “D” groups were first detected. During the course of ontogeny a considerable increase of immunoreactive cells occurred and segregation of the large central forebrain cluster into several rostrally and laterally distributed “D” groups took place. Some “D” groups that occur in the adult brain were not present in the E19 embryo.This study provides a first report of the localization of several unique cell groups in the brain of rat embryos and their appearance at different stages of gestation. It also gives further support to the notion that variations of aromaticl-amino acid decar☐ylase staining intensities may be characteristic of different monoamine neurons.