Isolation and characterization of the laure olfactory behavioral mutant in the zebrafish, Danio rerio.
Isolation and characterization of the laure olfactory behavioral mutant in the zebrafish, Danio rerio.
复制标题
斑马鱼劳尔嗅觉行为突变体的分离和表征。
DOI：
10.1002/dvdy.20530
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发表时间：
2005
期刊：
影响因子：
--
通讯作者：
Whitlock,KE
中科院分区：
文献类型：
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作者：
Vitebsky,A;Reyes,R;Sanderson,MJ;Michel,WC;Whitlock,KE
为了启动斑马鱼嗅觉发育和功能的遗传分析，我们开发了一种影响嗅觉感觉系统的突变的行为遗传筛选。首先，我们描述了野生型斑马鱼对各种气味的嗅觉反应。我们发现，3天大的幼年斑马鱼对氨基酸L-半胱氨酸产生了厌恶的行为反应。我们分离出一个突变体，laure（lre），它在发育3天时对L-半胱氨酸没有表现出厌恶的行为反应，并对该突变体的缺陷进行了初步表征。我们发现，lremutant鱼也有缺陷，在他们的反应L-丝氨酸和L-丙氨酸，但不是牛磺胆酸，作为年轻的成年人。此外，lremutant鱼有显着减少初级嗅觉感觉神经元比正常的，这些神经元的轴突没有形成特征性的轴突终止模式在发展中的嗅球。然而，嗅觉上皮的frefromutant鱼表现出正常或接近正常的电生理反应的几种气味。我们的数据表明，在突变体中观察到的行为缺陷是由于嗅球内发育中的嗅觉感觉神经元及其轴突连接的中断。该突变体的分离表明，我们的基于行为的筛选代表了一种可行的方法，用于在该脊椎动物模型系统中进行嗅觉行为的遗传解剖。Developmental Dynamics 234：229-242，2005.© 2005 Wiley利斯公司
To initiate a genetic analysis of olfactory development and function in the zebrafish,Danio rerio, we developed a behavioral genetic screen for mutations affecting the olfactory sensory system. First, we characterized olfactory responses of wild‐type zebrafish to various odors. We found that 3‐day‐old juvenile zebrafish reacted to the amino acid L‐cysteine with an aversive behavioral response. We isolated one mutant,laure(lre), which showed no aversive behavioral response to L‐cysteine at 3 days of development, and carried out a preliminary characterization of this mutant's defects. We found thatlremutant fish were also defective in their response to L‐serine and L‐alanine, but not to taurocholic acid, as young adults. In addition,lremutant fish had significantly fewer primary olfactory sensory neurons than normal, and the axons of these neurons did not form the characteristic axon termination pattern in the developing olfactory bulb. Nevertheless, the olfactory epithelium oflremutant fish showed normal or near normal electrophysiological responses to several odorants. Our data suggest that the behavioral defects observed in thelremutant result from the disruption of the developing olfactory sensory neurons and their axonal connections within the olfactory bulb. The isolation of thelremutant shows that our behavior‐based screen represents a viable approach for carrying out a genetic dissection of olfactory behaviors in this vertebrate model system. Developmental Dynamics 234:229–242, 2005. © 2005 Wiley‐Liss, Inc.