Redistribution of phosphatidylethanolamine at the cleavage furrow of dividing cells during cytokinesis
Redistribution of phosphatidylethanolamine at the cleavage furrow of dividing cells during cytokinesis
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DOI：
10.1073/pnas.93.23.12867
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发表时间：
1996-11-12
影响因子：
11.1
通讯作者：
Umeda, M
中科院分区：
文献类型：
--
作者：
Emoto, K;Kobayashi, T;Umeda, M
Ro09-0198是一种由19个氨基酸组成的四环多肽，它严格识别磷脂酰乙醇胺(PE)的结构，并与PE在生物膜上形成紧密的等摩尔复合体。利用环肽结合荧光标记的链霉亲和素，我们分析了PE在中国仓鼠卵巢细胞分裂中的细胞表面定位。我们发现，在胞质分裂末期，PE仅在细胞分裂末期的裂沟处暴露在细胞表面，而在胞质分裂的末期，PE仅暴露在细胞表面，而质膜结合的环肽在细胞质分裂过程中的分布没有发生变化，这表明PE的表面暴露反映了PE在分裂沟的增强争抢。此外，在前中期细胞中加入环肽和链霉亲和素诱导的PE细胞表面固定化，有效地阻止了末期晚期的胞质分裂。多肽-链霉亲和素复合处理不影响微管的皱纹、微管的重排和核的重建，但特异性地抑制了分裂沟处肌动蛋白细丝的分解和随后的膜融合。这些结果表明，质膜磷脂的重新分布是细胞质分裂的关键步骤，细胞表面的PE可能在调节收缩环和质膜之间的协调运动以实现成功的细胞分裂中发挥关键作用。
Ro09-0198 is a tetracyclic polypeptide of 19 amino acids that recognizes strictly the structure of phosphatidylethanolamine (PE) and forms a tight equimolar complex with PE on biological membranes, Using the cyclic peptide coupled with fluorescence-labeled streptavidin, we have analyzed the cell surface localization of PE in dividing Chinese hamster ovary cells. We found that PE was exposed on the cell surface specifically at the cleavage furrow during the late telophase of cytokinesis, PE was exposed on the cell surface only during the late telophase and no alteration in the distribution of the plasma membrane-bound cyclic peptide was observed during the cytokinesis, suggesting that the surface exposure of PE reflects the enhanced scrambling of PE at the cleavage furrow. Furthermore, cell surface immobilization of PE induced by adding the cyclic peptide coupled with streptavidin to prometaphase cells effectively blocked the cytokinesis at late telophase. The peptide-streptavidin complex treatment had no effect on furrowing, rearrangement of microtubules, and nuclear reconstitution, but specifically inhibited both actin filament disassembly at the cleavage furrow and subsequent membrane fusion, These results suggest that the redistribution of the plasma membrane phospholipids is a crucial step for cytokinesis and the cell surface PE may play a pivotal role in mediating a coordinate movement between the contractile ring and plasma membrane to achieve successful cell division.