Evaluation and comparison of protein splicing by exogenous inteins with foreign exteins in Escherichia coli
Evaluation and comparison of protein splicing by exogenous inteins with foreign exteins in Escherichia coli
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DOI：
10.1016/j.febslet.2011.10.005
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发表时间：
2011-11-04
期刊：
影响因子：
3.5
通讯作者：
Iwai, Hideo
中科院分区：
文献类型：
--
作者：
Ellila, Simo;Jurvansuu, Jaana Maria;Iwai, Hideo
内含肽催化的蛋白质剪接已经使各种生物技术应用成为可能，例如蛋白质连接。内含肽的成功应用常常受到剪接效率的限制。在这里，我们报告了20种不同的内含肽之间的蛋白质剪接在相同的情况下，从不同的生物体，以确定强大的内含肽与外源外显肽的比较。我们发现，RadA内含肽从Pyrococcus horikoshii和工程DnaB内含肽从念珠藻表现出同样有效的剪接活性，以前报道的高效DnaE内含肽从念珠藻。新鉴定的具有高效顺式剪接活性的内含肽可以为进一步开发新的蛋白质工程工具提供良好的起点。(C)2011年欧洲生物化学学会联合会。由Elsevier B出版。版权所有© 2016
Protein splicing catalyzed by inteins has enabled various biotechnological applications such as protein ligation. Successful applications of inteins are often limited by splicing efficiency. Here, we report the comparison of protein splicing between 20 different inteins from various organisms in identical contexts to identify robust inteins with foreign exteins. We found that RadA intein from Pyrococcus horikoshii and an engineered DnaB intein from Nostoc punctiforme demonstrated an equally efficient splicing activity to the previously reported highly efficient DnaE intein from Nostoc punctiforme. The newly identified inteins with efficient cis-splicing activity can be good starting points for the further development of new protein engineering tools. (C) 2011 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.