Combination of solid-phase extraction with microextraction techniques followed by HPLC for simultaneous determination of 2-methylimidazole and 4-methylimidazole in beverages
Combination of solid-phase extraction with microextraction techniques followed by HPLC for simultaneous determination of 2-methylimidazole and 4-methylimidazole in beverages
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固相萃取与微萃取技术结合高效液相色谱法同时测定饮料中的2-甲基咪唑和4-甲基咪唑
DOI：
10.1016/j.foodchem.2019.125389
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发表时间：
2020
期刊：
影响因子：
8.8
通讯作者：
Yu Shujuan
中科院分区：
文献类型：
--
作者：
Wu Chunjian;Wang Li;Li He;Yu Shujuan
由于2-甲基咪唑和4-甲基咪唑具有致癌性，因此需要一种灵敏的分析方法来监测2-甲基咪唑和4-甲基咪唑。在这里，我们提出了一个高灵敏度的方法的基础上相结合的固相萃取和分散液-液微萃取技术，然后通过高效液相色谱法，同时测定饮料中的2-MI和4-MI。丹磺酰氯用作衍生化试剂。采用Plackett-Burman设计和响应面法优化微萃取工艺参数。结果表明，衍生化导致显着改善色谱行为的2-MI和4-MI由于增加的疏水性。该方法线性关系良好（R2≥ 0.9985），精密度（%RSD ≤ 8.3%），定量限低（20 ng/mL），用于碳酸饮料、啤酒和能量饮料中2-MI和4-MI的测定，回收率为85-101%。该方法为饮料中纳克/毫升水平的2-MI和4-MI的常规分析提供了可能。
A sensitive analytical method for the monitoring 2-methylimidazole and 4-methylimidazole (2-MI and 4-MI) is desirable due to their carcinogenic property. Here, we propose a highly sensitive method basing on the combination of solid-phase extraction and dispersive liquid-liquid microextraction techniques followed by high-performance liquid chromatography to simultaneously determine 2-MI and 4-MI in beverages. Dansyl chloride was used as a derivatizing reagent. Microextraction parameters were optimized by Plackett-Burman design and response surface methodology. Results show that derivatization led to significant improvements in chromatographic behavior for 2-MI and 4-MI due to increased hydrophobicity. The method shows good linearity (R2≥ 0.9985), satisfactory precision (%RSD ≤ 8.3%) and low limit of quantification (20 ng/mL), and was successfully applied to determine 2-MI and 4-MI in carbonated drinks, beers and energy drinks, achieving satisfactory recoveries (85–101%). This method provides a potential for routine analysis of 2-MI and 4-MI at the nanogram per milliliter level in beverages.