Development of nanocomposite scaffolds based on TiO2 doped in grafted chitosan/hydroxyapatite by freeze drying method and evaluation of biocompatibility
Development of nanocomposite scaffolds based on TiO2 doped in grafted chitosan/hydroxyapatite by freeze drying method and evaluation of biocompatibility
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DOI：
10.1016/j.ijbiomac.2017.03.067
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发表时间：
2017-08-01
影响因子：
8.2
通讯作者：
Saber-Samandari, Saeed
中科院分区：
文献类型：
--
作者：
Abd-Khorsand, Saber;Saber-Samandari, Samaneh;Saber-Samandari, Saeed
采用冷冻干燥技术制备了多孔三维骨支架，具有作为松质骨移植替代品的应用潜力。将不同重量比的羟基磷灰石包埋在聚丙烯酸接枝壳聚糖的网络中，并以TiO2作为辅助组分制备多孔纳米复合骨支架。采用傅里叶变换红外光谱、扫描电镜、能量色散x射线光谱、x射线衍射分析和力学测试对制备的支架进行表征。这些支架具有良好的控制和相互连接的多孔结构。选择适量的羟基磷灰石可有效调节支架的孔径和孔隙率。力学性能测量结果表明，支架在干燥状态下基本保持强度，具有接近松质骨的力学性能。在水和磷酸盐缓冲盐水溶液中也检测了支架的膨胀行为。MTT法测定了纳米复合材料支架对人成纤维胶质（HuGu）细胞的细胞毒性，实验时间为24、48和72 h。结果表明，制备的纳米复合材料支架适用于骨组织工程。(C) 2017 Elsevier B.V.版权所有
Porous three-dimensional scaffolds with potential for application as cancellous bone graft substitutes were prepared using the freeze-drying technique. Hydroxyapatite with different weight ratio was embedded in the network of poly(acrylic acid) grafted chitosan accompanied by using TiO2 as an auxiliary component to fabricate porous nanocomposite bone scaffolds. Fourier transform infrared spectroscopy, scanning electron microscopy, energy-dispersive X-ray spectroscopy, and X-ray diffraction analysis and mechanical tests were carried out to characterize the prepared scaffolds. These scaffolds showed well controlled and interconnected porous structures. The pore size and porosity of the scaffolds could be effectively modulated by selecting appropriate amounts of hydroxyapatite. The results obtained from mechanical properties measurements indicated that the scaffolds could basically retain their strength in their dry state and have adequate mechanical properties close to those of cancellous bone. The swelling behavior of the scaffolds was also examined in both water and phosphate buffer saline solution. The cytotoxicity of the scaffold was determined by MTT assays on human fibroblast gum (HuGu) cells for 24, 48 and 72 h. In conclusion, this investigation demonstrates that the fabricated nanocomposite scaffolds are suitable for bone tissue engineering. (C) 2017 Elsevier B.V. All rights reserved.