Membrane-thinning effect of curcumin
Membrane-thinning effect of curcumin
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DOI：
10.1529/biophysj.107.126888
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发表时间：
2008-06-01
影响因子：
3.4
通讯作者：
Huang, Huey W.
中科院分区：
文献类型：
--
作者：
Hung, Wei-Chin;Chen, Fang-Yu;Huang, Huey W.
姜黄素与脂质双层的相互作用还没有很好的理解。最近的一项实验表明，姜黄素显着影响短杆菌肽在1，2-二油酰基-sn-甘油-3-磷酸胆碱（DOPC）双层的单通道寿命，而不影响其单通道电导。我们做了两个实验来理解这个结果。用等温滴定量热法测定了姜黄素与DOPC双层膜结合的分配系数。通过X射线片层衍射，我们测量了DOPC双层的厚度变化作为姜黄素/脂质比的函数。发现姜黄素具有非线性的膜减薄效应。通过等温滴定量热法和X-射线结果的组合，短杆菌肽的数据进行了定性解释。我们发现姜黄素不仅使脂质双层变薄，还可能削弱其弹性模量。结果表明，姜黄素可能通过改变宿主细胞膜的性质来影响膜蛋白的功能。
Interaction of curcumin with lipid bilayers is not well understood. A recent experiment showed that curcumin significantly affected the single-channel lifetime of gramicidin in a 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) bilayer without affecting its single-channel conductance. We performed two experiments to understand this result. By isothermal titration calorimetry, we measured the partition coefficient of curcumin binding to DOPC bilayers. By x-ray lamellar diffraction, we measured the thickness change of DOPC bilayers as a function of the curcumin/lipid ratio. A nonlinear membrane-thinning effect by curcumin was discovered. The gramicidin data were qualitatively interpreted by the combination of isothermal titration calorimetry and x-ray results. We show that not only does curcumin thin the lipid bilayer, it might also weaken its elasticity moduli. The result implies that curcumin may affect the function of membrane proteins by modifying the properties of the host membrane.