Chimeric RNA Oligonucleotides with Triazole and Phosphate Linkages: Synthesis and RNA Interference
Chimeric RNA Oligonucleotides with Triazole and Phosphate Linkages: Synthesis and RNA Interference
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DOI：
10.1002/asia.201500765
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发表时间：
2015-12-01
影响因子：
4.1
通讯作者：
Isobe, Hiroyuki
中科院分区：
文献类型：
--
作者：
Fujino, Tomoko;Kogashi, Kanako;Isobe, Hiroyuki
使用溶液相点击化学和固相自动化合成合成具有人工三唑接头的嵌合RNA寡核苷酸。已经开发了用于嵌合结构的接合单元的可扩展的合成方法，并且在接合单元与三唑接头点击偶联之后，通过利用成熟的亚磷酰胺方法进行延伸来制备一系列嵌合寡核苷酸。在不同链和位置具有三唑接头的一系列嵌合21-mer寡核苷酸允许RNA干扰的筛选研究，以澄清小干扰RNA分子中三唑修饰的偏好。
Chimeric RNA oligonucleotides with an artificial triazole linker were synthesized using solution-phase click chemistry and solid-phase automated synthesis. Scalable synthesis methods for jointing units for the chimeric structure have been developed, and after click-coupling of the jointing units with triazole linkers, a series of chimeric oligonucleotides was prepared by utilizing the well-established phosphoramidite method for the elongation. The series of chimeric 21-mer oligonucleotides that possessed the triazole linker at different strands and positions allowed for a screening study of the RNA interference to clarify the preference of the triazole modifications in small-interfering RNA molecules.