Protein tubule immobilization on self-assembled monolayers on Au substrates
Protein tubule immobilization on self-assembled monolayers on Au substrates
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DOI：
10.1021/nl015564v
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发表时间：
2001-09-01
期刊：
影响因子：
10.8
通讯作者：
Douberly, GE
中科院分区：
文献类型：
--
作者：
Matsui, H;Porrata, P;Douberly, GE
将抗生物素蛋白包被的肽小管（蛋白质小管）锚定在Au基底上的生物素掺入的自组装单分子层（SAM）上，并组装成SAM之间的桥梁。将图案化的生物素-SAM/Au基底与蛋白质小管一起置于柠檬酸溶液（pH 6）中。12 h后，亲和素小管被固定在生物素-自组装膜上，一些小管桥接图案化的生物素-自组装膜。未发现亲和素小管附着在生物素-SAM/Au基底之间的玻璃区域上。这一结果表明，蛋白质小管具有作为微电子连接线的潜力。
Avidin-coated peptide tubules, protein tubules, were anchored onto biotin-incorporated self-assembled monolayers (SAMs) on Au substrates and assembled as bridges between the SAMs. The patterned biotin-SAM/Au substrates were placed in a citric acid solution (pH 6) with the protein tubules. After 12 h, the avidin tubules were immobilized on the biotin-SAMs and some of the tubules bridged the patterned biotin-SAMs. No avidin tubules were found to attach onto the glass region between the biotin-SAM/Au substrates. This result shows that the protein tubules have potential to serve as connecting wires for microelectronics.