Viewing the bilayer hydrocarbon core using neutron diffraction.

Viewing the bilayer hydrocarbon core using neutron diffraction.
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使用中子衍射观察双层碳氢化合物核心。

DOI:
10.1007/s00232-008-9151-3
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发表时间:
2009
期刊:
The Journal of membrane biology
影响因子:
--
通讯作者:
Hristova,Kalina
Hristova,Kalina
中科院分区:
--
文献类型:
--
作者:
Han,Xue;Hristova,Kalina

文献摘要

被引文献

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膜蛋白在其天然流体脂质环境中折叠、组装和发挥作用。因此,流体脂质双层的结构研究对于理解膜中的过程至关重要。在这里,我们提出了一种使用中子衍射和市售氘化脂质来可视化碳氢化合物核心的简单方法。这种方法应该在膜中蛋白质插入和折叠的双层响应的结构研究中具有广泛的用途。
Membrane proteins fold, assemble and function within their native fluid lipid environment. Structural studies of fluid lipid bilayers are thus critically important for understanding processes in membranes. Here, we propose a simple approach to visualize the hydrocarbon core using neutron diffraction and deuterated lipids that are commercially available. This method should have broad utility in structural studies of the bilayer response to protein insertion and folding in membranes.