Inaccuracy of Single-Target Sequencing for Discriminating Species of the Mycobacterium abscessus Group
Inaccuracy of Single-Target Sequencing for Discriminating Species of the Mycobacterium abscessus Group
复制标题
DOI：
10.1128/jcm.00037-09
复制
发表时间：
2009-08-01
影响因子：
9.4
通讯作者：
Heym, Beate
中科院分区：
文献类型：
--
作者：
Macheras, Edouard;Roux, Anne-Laure;Heym, Beate
我们确定了59个临床分离株（来自58例患者）的分枝杆菌组的rpoB，hsp65和sodA的核苷酸序列。基于3个靶基因，对44个分离株（25 M. 15株具有“种间复合模式”的分离株的模式不一致。“对五个管家基因的序列分析也显示了这15个分离株中的8个的复合模式。
We determined nucleotide sequences of rpoB, hsp65, and sodA in 59 clinical isolates (from 58 patients) of the Mycobacterium abscessus group. Identification to the species level, based on three target genes, was concordant for 44 isolates (25 M. abscessus, 13 Mycobacterium massiliense, and 6 Mycobacterium bolletii isolates) and discordant for 15 isolates which had "interspecific composite patterns." Sequence analysis of five housekeeping genes also showed composite patterns in 8 of these 15 isolates.