Overexpression of a novel cold-responsive transcript factor LcFIN1 from sheepgrass enhances tolerance to low temperature stress in transgenic plants
Overexpression of a novel cold-responsive transcript factor LcFIN1 from sheepgrass enhances tolerance to low temperature stress in transgenic plants
复制标题
羊草中新型冷响应转录因子 LcFIN1 的过度表达可增强转基因植物对低温胁迫的耐受性。
DOI：
10.1111/pbi.12435
复制
发表时间：
2016-03-01
影响因子：
13.8
通讯作者：
Cheng, Liqin
中科院分区：
文献类型：
--
作者：
Gao, Qiong;Li, Xiaoxia;Cheng, Liqin
羊草（Leymus chinensis（Trin.）Tzvel）对低温胁迫具有很强的耐受性。之前的报告表明，绵羊草能够忍受低至-47.5摄氏度，使其能够在寒冷的冬季生存。然而，由于缺乏足够的研究，导致异常低温耐受性的潜在机制尚不清楚。虽然转录谱分析提供了深入了解转录组对冷胁迫的响应，但需要更详细的研究来剖析关于优异的非生物胁迫耐受性的分子机制。本文报道了一个新的绵羊草转录因子LcFIN 1（L.chinensis freezing-induced 1）。LcFIN 1与其他已知基因没有同源性，但在低温胁迫下被快速诱导，表明LcFIN 1参与了低温胁迫的早期反应。LcFIN 1的异位表达显著提高了转基因植株的抗寒性，表现为冷冻处理后植株的存活率、鲜重和其他胁迫相关指标均有所提高。转录组分析表明，LcFIN 1基因在过表达的植物中表达差异，表明LcFIN 1基因可能通过转录调控增强植物的非生物胁迫耐受性。电泳迁移率变动分析和CHIP-qPCR结果表明，LcCBF 1可以与LcFIN 1启动子区的CRT/DRE顺式元件结合，表明LcFIN 1受LcCBF 1直接调控。综上所述，我们的研究结果表明，LcFIN 1正调控植物对冷胁迫的适应反应，是一个有希望的候选基因，以提高作物的耐冷性。
As a perennial forage crop broadly distributed in eastern Eurasia, sheepgrass (Leymus chinensis (Trin.) Tzvel) is highly tolerant to low-temperature stress. Previous report indicates that sheepgrass is able to endure as low as -47.5 degrees C,allowing it to survive through the cold winter season. However, due to the lack of sufficient studies, the underlying mechanism towards the extraordinary low-temperature tolerance is unclear. Although the transcription profiling has provided insight into the transcriptome response to cold stress, more detailed studies are required to dissect the molecular mechanism regarding the excellent abiotic stress tolerance. In this work, we report a novel transcript factor LcFIN1 (L.chinensis freezing-induced 1) from sheepgrass. LcFIN1 showed no homology with other known genes and was rapidly and highly induced by cold stress, suggesting that LcFIN1 participates in the early response to cold stress. Consistently, ectopic expression of LcFIN1 significantly increased cold stress tolerance in the transgenic plants, as indicated by the higher survival rate, fresh weight and other stress-related indexes after a freezing treatment. Transcriptome analysis showed that numerous stress-related genes were differentially expressed in LcFIN1-overexpressing plants, suggesting that LcFIN1 may enhance plant abiotic stress tolerance by transcriptional regulation. Electrophoretic mobility shift assays and CHIP-qPCR showed that LcCBF1 can bind to the CRT/DRE cis-element located in the promoter region of LcFIN1, suggesting that LcFIN1 is directly regulated by LcCBF1. Taken together, our results suggest that LcFIN1 positively regulates plant adaptation response to cold stress and is a promising candidate gene to improve crop cold tolerance.