Detection of serum IgE antibody directed to aminothiazole using immobilized cephalosporins without protein conjugation
Detection of serum IgE antibody directed to aminothiazole using immobilized cephalosporins without protein conjugation
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使用无需蛋白缀合的固定化头孢菌素检测针对氨基噻唑的血清 IgE 抗体
DOI：
10.1046/j.1440-1592.1999.00149.x
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发表时间：
1999
期刊：
影响因子：
--
通讯作者：
K. Hiwada
中科院分区：
文献类型：
--
作者：
A. Yokoyama;N. Kohno;Kimiko Sakai;Hitoshi Katayama;K. Irifune;K. Kondo;Y. Hirasawa;K. Hiwada
It is well known that allergic reactions may sometimes occur in patients under treatment with β-lactam antibiotics. For the detection of antidrug antibodies in vitro, conjugation with human serum albumin has been considered to be essential. In this study, we found that cefotiam, cefpirome, and ceftazidime could be immobilized without conjugation to carrier protein to construct a solid-phase enzyme-linked immunosorbent assay (ELISA) system. We describe a patient (26-year-old female nurse) with contact urticaria induced by antibiotics. Using the serum of this patient, we successfully detected IgE antibody directed to the aminothiazolyl group of cephalosporins, which has not previously been reported. Results suggest that the simple ELISA using unconjugated antibiotics could be applicable to patients with allergy to some cephalosporins and the aminothiazole side chain of the cephalosporins could cause an IgE-mediated allergic reaction.
