Photoreceptor layer reconstruction in a rodent model of retinal degeneration
Photoreceptor layer reconstruction in a rodent model of retinal degeneration
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DOI：
10.1006/exnr.1999.7157
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发表时间：
1999-09-01
影响因子：
5.3
通讯作者：
Lund, RD
中科院分区：
文献类型：
--
作者：
Kwan, ASL;Wang, S;Lund, RD
我们已经研究了视网膜细胞移植到营养不良性视网膜变性小鼠身上的可能性，作为一种替代由于固有遗传缺陷而丢失的光感受器的方法。出生后早期轻度分离的视网膜在移植到视网膜下腔后存活至少6周。在一个重要的分布区域，移植细胞形成了与宿主视网膜色素上皮细胞层紧密对接的外部节段。移植物与剩余的宿主视网膜整合在一起，至少足以调节简单的明暗偏好。在移植物-宿主交界处可以看到一个新的突触层，其中包含大量的光感受器突触。这一点以及在低亮度水平下可以引发这种行为的事实，证明了移植细胞和宿主视网膜之间的功能回路重建，(C)1999年学术出版社。
We have examined the potential of retinal cell transplantation to dystrophic retinal degeneration mice as a way of replacing photoreceptors lost because of an intrinsic genetic defect. Early postnatal retinae which had been gently dissociated survived for at least 6 weeks after transplantation to the subretinal space. Over a significant area of distribution, transplanted cells formed outer segments which lay in close apposition to the host retinal pigment epithelial cell layer. The grafts integrated with the remaining host retina, sufficient at least to mediate a simple light-dark preference. A new synaptic layer was seen at the graft-host interface, which contained substantial numbers of photoreceptor synapses. This and the fact that the behavior could be elicited at low luminance levels argue for functional circuit reconstruction between grafted cells and host retina, (C) 1999 Academic Press.