Rapid and label-free detection and assessment of bacteria by terahertz time-domain spectroscopy
Rapid and label-free detection and assessment of bacteria by terahertz time-domain spectroscopy
复制标题
通过太赫兹时域光谱快速、无标记地检测和评估细菌
DOI：
10.1002/jbio.201500270
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发表时间：
2016-10-01
影响因子：
2.8
通讯作者：
Fu, Weiling
中科院分区：
文献类型：
--
作者：
Yang, Xiang;Wei, Dongshan;Fu, Weiling
在这里，我们展示了太赫兹（THz）光谱检测传染病中常见的四种细菌的潜力和适用性。除了细菌种类之间的光谱特性差异外，还研究了活细菌、死细菌和同种细菌粉末的THz吸收差异。我们的研究结果表明，细菌细胞之间的含水量的微小差异解释了吸收系数的明显差异，这可以用于细菌物种鉴定。此外，活的和死的细菌由于其不同的水合水平而显示出不同的吸收系数，这表明THz光谱可以用于快速评估被测细菌的存活状态。我们的研究结果清楚地证明了太赫兹光谱技术能够以最少的样品制备来节省时间和无标记地检测细菌，在不久的将来可能用于即时检测。[图形]通过THz光谱进行细菌检测的示意图。不同的细菌由于其含水量不同而具有不同的吸收系数。
Here we demonstrated the potential and applicability of terahertz (THz) spectroscopy to detect four commonly found bacteria in the infectious diseases. Besides the different spectral characteristics between bacterial species, THz absorption differences for living bacteria, dead bacteria and bacterial powder of the same species were also investigated. Our results revealed that small differences in water contents between bacterial cells account for distinct discrepancies of the absorption coefficients, which can be used for bacterial species identification. Furthermore, living and dead bacteria showed different absorption coefficients as a result of their different hydration levels, suggesting that THz spectroscopy can be used to rapidly assess the living state of bacteria under test. Our results clearly demonstrated the ability of THz spectroscopy for time-saving and label-free detection of bacteria with minimal sample preparation, potentially to be utilized for point-of-care tests in the near future.[GRAPHICS]Schematic representation of bacterial detection by THz spectroscopy. Different bacteria have distinctive absorption coefficients as a result of their different water contents.