Hollow chitosan/poly(acrylic acid) nanospheres as drug carriers
Hollow chitosan/poly(acrylic acid) nanospheres as drug carriers
复制标题
空心壳聚糖/聚丙烯酸纳米球作为药物载体
DOI：
10.1021/bm0608176
复制
发表时间：
2007-04-01
影响因子：
6.2
通讯作者：
Yang, Changzheng
中科院分区：
文献类型：
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作者：
Hu, Yong;Ding, Yin;Yang, Changzheng
研究了负载阿霉素（DOX）的壳聚糖（CS）-聚丙烯酸（PAA）空心纳米球的制备、体外释放、体外细胞毒性和体内药物递送。通过将一定量的DOX溶解在非交联的CS-PAA纳米球水溶液中，然后用戊二醛交联壳聚糖来完成负载。载药量高达4.3%，动态光散射测定载药空心纳米球粒径为118 nm。纳米球在体外显示出包埋的 DOX 持续释放长达 10 天，并且与游离 DOX 相比，对 HepG2 细胞显示出相当的体外细胞毒性。负载DOX的CS-PAA纳米球的体内DOX递送表明，与游离DOX溶液相比，血液中的DOX浓度可以维持更长的时间，并且小鼠肝脏中的DOX浓度可以持续维持在较高水平。负载DOX的CS-PAA中空纳米球的有趣特征是负载的DOX可以被递送到小鼠大脑中。共聚焦激光扫描显微镜分析表明，异硫氰酸荧光素（FITC）标记的CS-PAA可以沉积在包括肝脏、脾脏和大脑在内的不同器官中。
The preparation, in-vitro release, in-vitro cytotoxicity, and in-vivo drug delivery of doxorubicin (DOX)-loaded chitosan (CS)-poly(acrylic acid) (PAA) hollow nanospheres were investigated. The loading was done by dissolving a certain amount of DOX in non-cross-linked CS-PAA nanospheres aqueous solution followed by cross-linking chitosan with glutaraldehyde. The drug-loading content was up to 4.3% and the size of drug-loaded hollow nanospheres, determined by dynamic light scattering, was 118 nm. The nanospheres showed a continuous release of the entrapped DOX up to 10 days in vitro and showed comparable in-vitro cytotoxicity against HepG2 cells compared to the free DOX. In-vivo DOX delivery of DOX-loaded CS-PAA nanospheres showed that DOX concentration in blood can be maintained for a longer period than free DOX solution, and the DOX concentration in mice liver can be maintained constantly at relatively high level. The interesting feature of DOX-loaded CS-PAA hollow nanopspheres is that the loaded DOX can be delivered into the mice brain. The confocal laser scanning microscopy analysis reveals that fluorescein isothiocyanate (FITC)-labeled CS-PAA can deposit in different organs including liver, spleen, and brain.