Biofilm formation, adherence, and hydrophobicity of M. sympodialis, M. globosa, and M. slooffiae from clinical isolates and normal skin Virulence factors of M. sympodialis, M. globosa and M. slooffiae
Biofilm formation, adherence, and hydrophobicity of M. sympodialis, M. globosa, and M. slooffiae from clinical isolates and normal skin Virulence factors of M. sympodialis, M. globosa and M. slooffiae
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DOI：
10.1093/mmy/myaa017
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发表时间：
2020-11-01
期刊：
影响因子：
2.9
通讯作者：
Giusiano, Gustavo
中科院分区：
文献类型：
--
作者：
Angiolella, Letizia;Rojas, Florencia;Giusiano, Gustavo
马拉色菌属由一组引起相似病理的异质物种组成。马拉色菌属酵母被认为是总皮肤真菌生物群中最丰富的皮肤真核生物。这种真菌的定殖或感染能力是由真菌细胞及其毒力因子之间的复杂相互作用决定的。本研究的目的是在体外评估的疏水性水平，在聚苯乙烯表面上的粘附能力和形成生物膜的能力，包括M。sympodialis、合柄霉M. globosa和M.来自健康受试者和来自皮肤病学病症的Slooffiae。细胞表面疏水性水平用两相系统测定。通过四唑盐（XTT）还原试验和扫描电子显微镜（SEM）测定生物膜形成。在所有研究的毒力因子中均观察到菌株依赖性。所有M. sympodialis、合柄霉M. globosa和M. Slooffiae证明了它们在可变容量下形成生物膜的能力。SEM观察证实了生物膜形成48小时后的可变细胞外基质。所有M. globosa是高度粘附的和/或疏水的以及生物膜生产者。相比之下，M。slooffiae是最少的生物膜生产者。结果表明，M.合足菌，作为临床分离物或作为人类微生物群的居民。本工作的结果与以前的M.研究证实，最常见的马拉色菌属分离自正常受试者的皮肤和皮肤病患者，形成具有不同能力的生物膜。这些毒力因子的研究是重要的，突出马拉色菌属物种之间的差异，并确定其参与病理过程。
The genus Malassezia comprises a heterogeneous group of species that cause similar pathologies. Malassezia yeasts were considered as the most abundant skin eukaryotes of the total skin mycobiome. The ability of this fungus to colonize or infect is determined by complex interactions between the fungal cell and its virulence factors. This study aims to evaluate in vitro the hydrophobicity levels, the adherence capacity on a polystyrene surface and the ability to form biofilm of 19 isolates, including M. sympodialis, M. globosa, and M. slooffiae, from healthy subjects and from dermatological disorders. Cellular surface hydrophobicity levels were determined by two-phase system. The biofilm formation was determined by tetrazolium salt (XTT) reduction assay and by Scanning Electron Microscopy (SEM). Strain dependence was observed in all virulence factors studied. All isolates of M. sympodialis, M. globosa, and M. slooffiae demonstrated their ability to form biofilm at variable capacities. SEM observations confirmed a variable extracellular matrix after 48 hours of biofilm formation. All isolates of M. globosa were highly adherent and/or hydrophobic as well as biofilm producers. In contrast, M. slooffiae was the least biofilm producer. No significant differences between virulence factors were demonstrated for M. sympodialis, either as clinical isolate or as inhabitant of human microbiota. Results of this work together with the previous M. furfur research confirm that the most frequently Malassezia species isolated from normal subject's skin and patients with dermatosis, form biofilm with different capacities. The study of these virulence factors is important to highlight differences between Malassezia species and to determine their involvement in pathological processes.