Integrated analysis of competitive endogenous RNA networks in elder patients with non-small cell lung cancer.
Integrated analysis of competitive endogenous RNA networks in elder patients with non-small cell lung cancer.
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DOI：
10.1097/md.0000000000033192
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发表时间：
2023-03-10
期刊：
影响因子：
1.6
通讯作者：
中科院分区：
文献类型：
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肺癌是世界上最常见的癌症之一，也是癌症相关死亡的主要原因；非小细胞肺癌（NSCLC）约占所有肺癌病例的80%。本研究旨在构建一个竞争性内源性RNA （ceRNA）网络，并确定老年NSCLC患者的预后特征。我们从癌症基因组图谱中提取了老年NSCLC患者的数据，并鉴定了差异表达（DE）信使rna （mrna）、微rna （miRNAs）和长链非编码rna （lncRNAs）。通过基因本体和京都基因与基因组百科全书分析来研究demrna的功能。使用starBase、TargetScan、miRTarBase和miRanda预测rna之间的相互作用。使用Cytoscape 3.0版本构建并可视化lncRNA-miRNA-mRNA ceRNA网络。构建的ceRNA网络中DERNAs的表达水平与总生存率之间的关系使用R软件中的生存包进行测定。此外，我们还研究了另一个基因表达Omnibus队列，以从外部验证ceRNA网络。共鉴定出2865个demmrna、62个demirna和131个delncrna。失调的mrna在癌症相关的过程和途径中富集。利用38个mirna、61个lncrna和164个mrna构建了ceRNA网络。其中，3个lncrna、3个mirna和16个mrna与总生存密切相关。MIR99AHG-hsa-miR-31-5p-PRKCE轴已被确定为参与老年人非小细胞肺癌发展的潜在ceRNA网络。在GSE19804队列中对MIR99AHG-hsa- mir -31-5p-PRKCE轴进行外部验证显示，与正常肺组织相比，老年NSCLC患者肿瘤组织中PRKCE下调，MIR99AHG上调。该研究为lncRNA-miRNA-mRNA ceRNA网络提供了新的见解，并揭示了老年NSCLC患者诊断和预后的潜在生物标志物。
Lung cancer is one of the most prevalent cancers and the leading cause of cancer-related deaths worldwide; non-small cell lung cancer (NSCLC) comprises approximately 80% of all lung cancer cases. This study aimed to construct a competing endogenous RNA (ceRNA) network and identify prognostic signatures in elderly patients with NSCLC. We extracted data from elderly patients with NSCLC from The Cancer Genome Atlas and identified differentially expressed (DE) messenger RNAs (mRNAs), microRNAs (miRNAs), and long non-coding RNAs (lncRNAs). Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses were performed to investigate the functions of DEmRNAs. The interactions between RNAs were predicted using starBase, TargetScan, miRTarBase, and miRanda. Cytoscape version 3.0 was used to construct and visualize the lncRNA-miRNA-mRNA ceRNA network. The association between the expression levels of DERNAs in the constructed ceRNA network and overall survival was determined using the survival package in R software. Furthermore, another Gene Expression Omnibus cohort was studied to externally validate the ceRNA network. In total, 2865 DEmRNAs, 62 DEmiRNAs, and 131 DElncRNAs were identified. Dysregulated mRNAs are enriched in cancer-related processes and pathways. A ceRNA network was constructed using 38 miRNAs, 61 lncRNAs, and 164 mRNAs. Of these, 3 lncRNAs, 3 miRNAs, and 16 mRNAs were closely related to overall survival. The MIR99AHG-hsa-miR-31-5p-PRKCE axis has been identified as a potential ceRNA network involved in the development of NSCLC in elderly individuals. External validation of the MIR99AHG-hsa-miR-31-5p-PRKCE axis in the GSE19804 cohort showed that PRKCE was downregulated and that MIR99AHG was upregulated in the tumor tissues of elderly patients with NSCLC compared with normal lung tissues. This study provides novel insights into the lncRNA-miRNA-mRNA ceRNA network and reveals potential biomarkers for the diagnosis and prognosis of elderly patients with NSCLC.
DOI：
10.3779/j.issn.1009-3419.2016.02.03
发表时间：
2016-02
期刊：
Zhongguo fei ai za zhi = Chinese journal of lung cancer
影响因子：
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作者：
Zhao S;Li L;Qiu Z;Chen Y;Jing Y;Zhou Y;Li W
通讯作者：
Li W