Intestinal immunomodulatory activity of indigestible glucan in mice and its utilization by intestinal bacteria in vitro.
Intestinal immunomodulatory activity of indigestible glucan in mice and its utilization by intestinal bacteria in vitro.
复制标题
小鼠难消化葡聚糖的肠道免疫调节活性及其体外肠道细菌的利用。
DOI：
10.1016/j.jff.2021.104759
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发表时间：
2021
影响因子：
5.6
通讯作者：
Matsumoto K.
中科院分区：
文献类型：
--
作者：
Horinouchi A;Hirai H;Hirano R;Kurihara S;Takagi H;Matsumoto K.
研究了难消化葡聚糖（IG）（一种新开发的水溶性葡萄糖聚合物）对肠道健康的功能特性。在高直链玉米淀粉、低聚果糖和 IG 中，在 BALB/cA 小鼠为期 5 周的实验中，IG 显示出最强的肠道 IgA 诱导作用（每种饮食 3%）。接下来，将分离的 IG 样品（聚合度 (DP) ≥ 5（高 IG）和 DP ≤ 5（低 IG）部分）喂给 BALB/cA 小鼠 9 周（每种饮食 3%）。高 IG 摄入量立即增加粪便 IgA，而低 IG 摄入量则逐渐增加。高 IG 组的粪便粘蛋白显着增加，但低 IG 组则没有。为了确定肠道细菌对高 IG 和低 IG 的利用，使用 57 种人类肠道细菌菌株进行了体外测定。很少有细菌利用高 IG，而更多细菌，特别是双歧杆菌菌株，利用低 IG。这些结果表明，摄入 IG 通过增强肠道屏障功能和益生元作用，有益于肠道环境。
The functional properties of indigestible glucan (IG), a newly developed water-soluble glucose polymer, on intestinal health were investigated. Of high amylose cornstarch, fructooligosaccharides, and IG, IG showed the strongest intestinal IgA induction during the 5-week experiment with BALB/cA mice (3% per diet). Next, separated IG samples, degree of polymerization (DP) ≥ 5 (High-IG) and DP ≤ 5 (Low-IG) fractions, were fed to BALB/cA mice for 9 weeks (3% per diet). High-IG consumption immediately increased the fecal IgA, whereas Low-IG consumption gradually increased it. Fecal mucin was significantly increased in the High-IG group but not in the Low-IG group. To identify utilization of High-IG and Low-IG by intestinal bacteria,in vitroassays were performed using 57 human gut bacterial strains. Few bacteria utilized High-IG, whereas more bacteria, particularlyBifidobacteriumstrains, utilized Low-IG. These results indicate that IG ingestion benefits the intestinal environment via strengthening the intestinal barrier function and prebiotic effects.