Binaphthyl-based amphiphile as a reagent for dynamically modified silica and fluorescence detection in high-performance liquid chromatography
Binaphthyl-based amphiphile as a reagent for dynamically modified silica and fluorescence detection in high-performance liquid chromatography
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基于联萘的两亲物作为动态改性二氧化硅和高效液相色谱中荧光检测的试剂
DOI：
10.1016/0021-9673(94)80120-7
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发表时间：
1994
影响因子：
4.1
通讯作者：
B. Juskowiak
中科院分区：
文献类型：
--
作者：
B. Juskowiak
介绍了一种含二萘表面活性剂对裸二氧化硅的改性。报道了几种因素对双苯基两亲体在二氧化硅表面吸附量的影响，并讨论了溶质的滞留机理。非离子型芳香族化合物的分离表现出明显的反相机制，即使在极低的二氧化硅覆盖率下（低于10 nmol m−2,3 μmol g−1），这表明吸附表面活性剂的联萘基部分与溶质的芳香族环之间存在很强的相互作用。离子对反相机制似乎是阴离子溶质保留的主要过程，特别是在高两亲性物质浓度的流动相中。讨论了荧光两亲体在液相色谱中的应用概念，并给出了利用“可视化效应”检测非荧光分析物的实例。
The modification of bare silica with a binaphthyl-containing surfactant is described. The effects of several factors on the amount of binaphthyl amphiphile adsorbed on the silica surface are reported and the retention mechanisms of solutes are discussed. The separation of non-ionic aromatic compounds shows a clear reversed-phase mechanism, even at very low coverage of silica (below 10 nmol m−2, 3 μmol g−1), which indicates an extremely strong interaction between binaphthyl moieties of the adsorbed surfactant and aromatic rings of the solutes. The ion-pair reversed-phase mechanism appears to be major process responsible for the retention of anionic solutes, especially at higher amphiphile concentrations in mobile phase. The concept of the application of fluorescent amphiphiles in liquid chromatography is discussed, and an example of the detection of non-fluorescent analytes using the “visualization effect” is also presented.