Estuarine sediment hydrocarbon-degrading microbial communities demonstrate resilience to nanosilver
Estuarine sediment hydrocarbon-degrading microbial communities demonstrate resilience to nanosilver
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DOI：
10.1016/j.ibiod.2014.09.004
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发表时间：
2014-12-01
影响因子：
4.8
通讯作者：
Whitby, Corinne
中科院分区：
文献类型：
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作者：
Beddow, Jessica;Stoelpe, Bjoern;Whitby, Corinne
目前对银纳米颗粒(AgNPs)对河口微生物群落的潜在影响知之甚少。英国科尔纳河口很容易受到船只交通造成的石油污染，附近的污水处理厂排放的污水可能会使AgNP暴露。本研究考察了无封顶AgNPs(UAgNPs)、封顶AgNPs(CAgNPs)和溶解Ag2SO4对河口沉积物中烃类降解微生物群落的影响。UAgNPs、cAgNPs和Ag2SO4(高达50 mg L-1)对碳氢化合物的生物降解没有显著影响(所有样品到第7天都有80%-92%的碳氢化合物被生物降解)。尽管经石油修正的沉积物中的细胞总数和活跃细胞数不受银暴露的影响；到第14天，加入50 mg L-1cAgNPs和0.5 mg L-1Ag2SO4的非油污沉积物中细胞总数分别从1.66和0.66×10(-7)g(-1)减少到0.66×10(-7)g(-1)。所有暴露于银的沉积物中的细菌群落结构也发生了显著的变化，与非银对照相比，50 mg L-1Ag2SO4暴露的非油污微生态系统中与类杆菌相对应的一条DGGE条带更加明显。总之，AgNPs似乎不会影响微生物对碳氢化合物的降解，但会影响细菌群落的多样性，这可能会对河口其他重要的微生物中介过程产生潜在影响。(C)2014年提交人。爱思唯尔有限公司出版。
Little is currently known about the potential impact of silver nanoparticles (AgNPs) on estuarine microbial communities. The Colne estuary, UK, is susceptible to oil pollution through boat traffic, and there is the potential for AgNP exposure via effluent discharged from a sewage treatment works located in close proximity. This study examined the effects of uncapped AgNPs (uAgNPs), capped AgNPs (cAgNPs) and dissolved Ag2SO4, on hydrocarbon-degrading microbial communities in estuarine sediments. The uAgNPs, cAgNPs and Ag2SO4 (up to 50 mg L-1) had no significant impact on hydrocarbon biodegradation (80-92% hydrocarbons were biodegraded by day 7 in all samples). Although total and active cell counts in oil-amended sediments were unaffected by silver exposure; total cell counts in non-oiled sediments decreased from 1.66 to 0.84 x 10(7) g(-1) dry weight sediment (dws) with 50 mg L-1 cAgNPs and from 1.66 to 0.66 x 10(7) g(-1) dws with 0.5 mg L-1 Ag2SO4 by day 14. All silver-exposed sediments also underwent significant shifts in bacterial community structure, and one DGGE band corresponding to a member of Bacteroidetes was more prominent in non-oiled microcosms exposed to 50 mg L-1 Ag2SO4 compared to non-silver controls. In conclusion, AgNPs do not appear to affect microbial hydrocarbon-degradation but do impact on bacterial community diversity, which may have potential implications for other important microbial-mediated processes in estuaries. (C) 2014 The Authors. Published by Elsevier Ltd.