Localization of estrone sulfatase in human breast carcinomas
Localization of estrone sulfatase in human breast carcinomas
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雌酮硫酸酯酶在人类乳腺癌中的定位
DOI：
10.1007/bf02966449
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发表时间：
1999
期刊：
影响因子：
4
通讯作者：
D. Salomon
中科院分区：
文献类型：
--
作者：
T. Saeki;S. Takashima;H. Sasaki;N. Hanai;D. Salomon
我们制备了抗人EL-STS的单抗，以定位人类乳腺癌中的雌酮硫酸酯酶(E1-STS)。特别是，我们通过免疫组织化学或Western blotting分析了MCF-7克隆E3、ZR-75-1、MDA-MB 231和MDA-MB-468乳腺癌细胞系以及25例乳腺癌。同时对乳腺组织的组织学资料、雌激素受体(ER)、孕激素受体(PGR)和表皮生长因子受体(EGFR)进行分析。均为女性患者的手术标本。25例癌中有9例发生在绝经前，16例发生在绝经后。所有细胞系均呈E1-STS阳性染色。有趣的是，在细胞膜上观察到了E1-STS的细小颗粒染色。此外，无论是否存在E2，Western blotting分析在所有乳腺癌细胞系中都检测到带有一条E1-STS特异性条带的65kD蛋白。25例癌组织中有22例(88.0%)E1-STS呈阳性表达，而癌旁间质呈阴性表达。绝经前癌组织中有8例(80.0%)表达阳性，而绝经后癌组织中有14例(93.3%)表达阳性。E1-STS在绝经后患者中的表达频率相对高于绝经前患者，但无统计学意义。E1-STS的免疫染色强度取决于肿瘤的大小(NS)。E1-STS的表达与其他指标均无相关性。这一证据表明，E1-STS的表达可能参与了乳腺癌的发生发展。E1-STS的表达与预后因素的关系尚需进一步研究。在乳腺癌组织中，E1-STS可能存在于癌细胞中，而在癌旁组织中不表达。
We generated anti-human El-STS monoclonal antibodies to localize estrone sulfatase (E1-STS) in human breast carcinomas. In particular, we examined the MCF-7 clone E3, ZR-75-1, MDA-MB 231, and MDA-MB-468 breast cancer cell lines and 25 breast carcinomas by either immunohistochemistry or Western blotting analysis. Simultaneously, we analyzed histological data, estrogen receptor (ER) status, progesterone receptor (PgR) status and epidermal growth factor receptor (EGFR) in breast tissue. All were surgical specimens from female patients. Nine of 25 carcinomas were obtained from premenopausal women, and 16 carcinomas were obtained from postmenopausal women. All cell lines demonstrated positive staining for E1-STS. Interestingly, fine granulated staining of E1-STS on the cell membrane was observed. In addition, Western blotting analysis detected a 65 kD protein with an E1-STS specific band in all breast cancer cell lines regardless of the presence or absence of E2. Twenty-two of 25 (88.0%) carcinomas showed positive staining for E1-STS, whereas negative staining was observed in the interstitial tissue surrounding tumors. In the premenopausal patients, 8 of 10 carcinomas (80.0%) showed positive staining for E1-STS, whereas 14 of 15 carcinomas (93.3%) revealed positive staining in the postmenopausal patients. The frequency of E1-STS expression was relatively higher in postmenopausal patients than in premenopausal patients but not statistically significant. The intensity of immunostaining for E1-STS depended upon the size of the tumor (NS). There was no correlation between E1-STS expression and other parameters. This evidence suggests E1-STS expression may be involved in the development of breast cancer. Further studies are necessary to clarify the relationship between E1-STS expression and prognostic factors. Immunoreactive E1-STS may be localized in cancer cells but not in surrounding tissues in breast cancer.
影响因子：
7.1
作者：
SIITERI, PK
通讯作者：
SIITERI, PK