Phorbol diester synergistically stimulates agonist-induced lipoxygenase product formation in murine macrophages.
Phorbol diester synergistically stimulates agonist-induced lipoxygenase product formation in murine macrophages.
复制标题
佛波二酯协同刺激小鼠巨噬细胞中激动剂诱导的脂氧合酶产物的形成。
DOI：
10.1016/0006-291x(86)90714-x
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发表时间：
1986
影响因子：
3.1
通讯作者：
Baxter,CS
中科院分区：
文献类型：
--
作者：
Wey,HE;Baxter,CS
将小鼠腹腔巨噬细胞与12-O-十四酰基佛波醇-13-乙酸酯（100 ng/ml）预孵育，然后暴露于各种浓度的钙离子载体A23187。这种联合治疗导致花生四烯酸代谢物的释放增加，这在本质上是协同的。释放到培养基中的[3 H]产物的解析显示a）对环加氧酶产物6-酮PGF 1和PGE 2的影响是加和的，小于加和的（A23187浓度依赖性），B）脂氧合酶产物的产生是协同增强的，和c）最大的影响是对12-HETE的产生。
Murine peritoneal macrophages were preincubated with 12-O-tetradecanoylphorbol-13-acetate (100 ng/ml) and then exposed to various concentrations of calcium ionophore A23187. This combined treatment resulted in an elevated release of arachidonic acid metabolites that was synergistic in nature. Resolution of the [3 H] products released into the medium revealed that a) the effect on the cyclooxygenase products 6-keto PGF 1 and PGE 2 were additive to less than additive (A23187 concentration dependent), b) the production of lipoxygenase products was synergistically enhanced, and c) the largest effect was on 12-HETE production.