Calbindin D-28K and parvalbumin expression in embryonic chick hippocampus is enhanced by prenatal auditory stimulation
Calbindin D-28K and parvalbumin expression in embryonic chick hippocampus is enhanced by prenatal auditory stimulation
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DOI：
10.1016/j.brainres.2007.11.021
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发表时间：
2008-01-29
期刊：
影响因子：
2.9
通讯作者：
Wadhwa, Shashi
中科院分区：
文献类型：
--
作者：
Chaudhury, Sraboni;Nag, Tapas Chandra;Wadhwa, Shashi
钙结合蛋白 (CaBP) 缓冲过量的胞质 Ca2+，胞质 Ca2+ 伴随外部刺激后的神经元活动。物种特异性声音和音乐的产前听觉刺激会影响雏鸡听觉通路的早期成熟和行为反应。在这项研究中，我们确定了产前听觉刺激后发育中的小鸡海马体的体积、神经元总数、钙结合蛋白 D-28K 和小清蛋白阳性神经元的比例及其表达水平。从胚胎第 10 天 (E) 直至孵化，家养雏鸡的受精卵全天候暴露在 6S dB 的物种特异性叫声或西塔琴音乐中 15 分钟/小时。检查发育阶段（E12、E16 和 E20）的海马。随着正常发育过程中胚胎年龄的增加，海马体的体积、神经元总数以及神经元比例和钙结合蛋白 D-28K 和小清蛋白的表达水平均增加。与年龄匹配的对照组相比，仅音乐刺激组在 E20 时的音量显着增加（p
Calcium-binding proteins (CaBPs) buffer excess of cytosolic Ca2+, which accompanies neuronal activity following external stimuli. Prenatal auditory stimulation by species-specific sound and music influences early maturation of the auditory pathway and the behavioral responses in chicks. In this study, we determined the volume, total number of neurons, proportion of calbindin D-28K and parvalbumin-positive neurons along with their levels of expression in the developing chick hippocampus following prenatal auditory stimulation. Fertilized eggs of domestic chicks were exposed to sounds of either species-specific calls or sitar music at 6S dB for 15 min/h round the clock from embryonic day (E) 10 until hatching. Hippocampi of developmental stages (E12, E16 and E20) were examined. With an increase in embryonic age during normal development, the hippocampus showed an increase in its volume, total number of neurons as well as in the neuron proportions and levels of expression of calbindin D-28K and parvalbumin. A significant increase of volume at E20 was noted only in the music-stimulated group compared to that of their age-matched control (p