Immunochemical determination of dioxins in sediment and serum samples
Immunochemical determination of dioxins in sediment and serum samples
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DOI：
10.1016/j.talanta.2004.05.030
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发表时间：
2004-08-08
期刊：
影响因子：
6.1
通讯作者：
Hammock, BD
中科院分区：
文献类型：
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作者：
Nichkova, M;Park, EK;Hammock, BD
多氯二苯并对二恶英(PCDDs)和多氯二苯并呋喃(PCDFs)被认为是剧毒污染物，这些化合物的环境和生物监测引起了极大的关注。免疫分析可以作为筛查方法，以满足日益增长的快速和低成本分析的需求。在这项工作中，我们描述了一种以2，3，7-三氯-8-甲基二苯并-对二恶英(TMDD)作为2，3，7，8-四氯二苯并-对二恶英(TCDD)的替代标准的免疫分析方法在沉积物和人体血清样品中的应用。为了建立最简单、最经济、最有效地消除酶联免疫吸附试验(ELISA)中的基质干扰的方法，建立了样品提取和制备方法。沉积物的整体方法是基于正己烷提取；通过多层硅胶柱和活性碳柱净化；有机溶剂交换与DMSO-Triton X-100和酶联免疫吸附测定。气相色谱-高分辨率质谱仪(GC-HRMS)验证研究(n=13)表明，该方法适用于沉积物中二恶英毒性当量(TEQ)的筛选，方法检出限约为100pgg(-1)干沉积物，精密度为13-33%RSD。需要对来自不同来源的大量样品进行分析，以更准确地确定筛选水平，以及沉积物免疫分析二恶英TEQ的假阳性和阴性数量。与GC-HRMS相比，用于沉积物分析的免疫分析方法在速度、样品吞吐量和成本方面都有改进。血清样品经简单的液液萃取和DMSO-Triton X-100溶剂交换后，无需进一步稀释即可测定二恶英。目前的方法(近似方法LOQ为200pgml(-1)血清)对于测定血清中的二恶英不够灵敏，无法测量可接受的接触限值。(C)2004爱思唯尔B.V.保留所有权利。
Polychlorinated dibenzo-p-dioxins (PCDDs) and polychlorinated dibenzofurans (PCDFs) are considered highly toxic contaminants and the environmental and biological monitoring of these compounds is of great concern. Immunoassays may be used as screening methods to satisfy the growing demand for rapid and low cost analysis. In this work, we describe the application of an immunoassay that uses 2,3,7-trichloro-8-methyldibenzo-p-dioxin (TMDD) as a surrogate standard for 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) to sediment and human serum samples. Sample extraction and preparation methods were developed with the aim to establish the simplest, cost-effective and efficient removal of the matrix interferences in the enzyme-linked immunosorbent assay (ELISA). The overall method for sediments is based on a hexane extraction; clean up by a multilayered silica gel column and an activated carbon column; an organic solvent exchange with DMSO-Triton X-100 and ELISA measurement. The gas chromatography-high resolution mass spectrometry (GC-HRMS) validation studies (n = 13) revealed that the method is suitable for the toxic equivalents (TEQ) screening of dioxin in sediments with a method detection limit of about 100 pg g(-1) dry sediment with a precision of 13-33% R.S.D. The analysis of a large number of samples originating from different sources would be required to establish more precisely the screening level, as well as the number of false positives and negatives of dioxin TEQ by the immunoassay for sediments. The immunoassay method for sediment analysis offers improvement in speed, sample throughput, and cost in comparison to GC-HRMS. Dioxins were determined in serum samples after a simple liquid-liquid extraction and solvent exchange into DMSO-Triton X-100 without further dilution. The current method (approximate method LOQ of 200 pg ml(-1) serum) is not sufficiently sensitive for the determination of dioxins in serum to measure acceptable exposure limit. (C) 2004 Elsevier B.V. All rights reserved.