Visualization of Mutant Aggregates from Clock Neurons by Agarose Gel Electrophoresis (AGERA) in Drosophila melanogaster.
Visualization of Mutant Aggregates from Clock Neurons by Agarose Gel Electrophoresis (AGERA) in Drosophila melanogaster.
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DOI：
10.1007/978-1-0716-2249-0_25
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发表时间：
2022-01-01
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影响因子：
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通讯作者：
Rosato, Ezio
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文献类型：
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作者：
Delfino, Laura;Campesan, Susanna;Rosato, Ezio
果蝇（Drosophila melanogaster）的时钟神经元已成为表达在几种人类神经退行性疾病中积累的错误折叠蛋白质聚集体的有用模型。这种方法的一个优点是，通过自动化、高度可靠和客观的程序，可以容易地对昼夜运动节律、睡眠或活动水平量化行为影响。因此，需要一种快速测定来可视化这些神经元是否形成聚集体。在这里，我们描述了一种改进的免疫印迹方法，琼脂糖凝胶电泳（AGERA），已被优化为解决聚集从苍蝇时钟神经元。
The clock neurons of the fruit fly Drosophila melanogaster have become a useful model for expressing misfolded protein aggregates that accumulate in several human neurodegenerative diseases. One advantage of such an approach is that the behavioral effects can be readily quantified on circadian locomotor rhythms, sleep or activity levels via automated, highly reliable and objective procedures. Therefore, a rapid assay is required to visualize whether these neurons develop aggregates. Here we describe a modified immunoblot method, agarose gel electrophoresis (AGERA) that has been optimized for resolving aggregates from fly clock neurons.