Analysis of mtDNA/nDNA Ratio in Mice.
Analysis of mtDNA/nDNA Ratio in Mice.
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DOI：
10.1002/cpmo.21
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发表时间：
2017-03-02
影响因子：
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通讯作者：
Auwerx J
中科院分区：
文献类型：
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作者：
Quiros PM;Goyal A;Jha P;Auwerx J
线粒体DNA（mtDNA）缺乏核DNA中核小体提供的保护，并且没有DNA修复机制，使其极易受到损伤，这可能导致mtDNA耗尽。线粒体DNA缺失损害细胞和组织的有效功能，从而对健康产生负面影响。在这里，我们描述了一个简单的协议，以定量mtDNA拷贝数，通过确定mtDNA/nDNA的比例，我们验证了在一个队列的年轻和老年小鼠。
The mitochondrial DNA (mtDNA) lacks a protection provided by the nucleosomes in the nuclear DNA and does not have a DNA repair mechanism, making it highly susceptible to damage, which can lead to mtDNA depletion. MtDNA depletion compromises the efficient function of cells and tissues and thus impacts negatively on health. Here, we describe a brief and easy protocol to quantify mtDNA copy number by determining the mtDNA/nDNA ratio that we validated in a cohort of young and aged mice.