ACTIVATED HUMAN-MONOCYTES EXPRESS THE C-SIS PROTOONCOGENE AND RELEASE A MEDIATOR SHOWING PDGF-LIKE ACTIVITY
ACTIVATED HUMAN-MONOCYTES EXPRESS THE C-SIS PROTOONCOGENE AND RELEASE A MEDIATOR SHOWING PDGF-LIKE ACTIVITY
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DOI：
10.1038/319158a0
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发表时间：
1986-01-09
期刊：
影响因子：
64.8
通讯作者：
CRYSTAL, RG
中科院分区：
文献类型：
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作者：
MARTINET, Y;BITTERMAN, PB;CRYSTAL, RG
目前关于伤口愈合机制和动脉粥样硬化、肺纤维化和肝纤维化的发病机制的观点表明单核吞噬细胞在吸引和/或刺激间充质细胞增殖中的核心作用1 -5。我们在这里证明，激活的人血单核细胞，而不是静息单核细胞，释放一种介质，吸引平滑肌细胞和其他介质合作，刺激成纤维细胞增殖。该介质与血小板衍生生长因子（PDGF）非常相似：其色谱特性和化学稳定性与PDGF相似6，7，其与125 I-PDGF竞争结合成纤维细胞，并且其与抗PDGF抗体免疫沉淀。同时，刺激的单核细胞，但不是休息的单核细胞，表达c-sis原癌基因，一个基因编码的PDGF chains 8，9，一致的概念，表达的c-sis原癌基因可能参与单核吞噬细胞的能力，以调节间充质细胞的积累。
Current ideas about the mechanism of wound healing and the pathogenesis of atherosclerosis, pulmonary fibrosis and hepatic fibrosis suggest a central role for the mononuclear phagocyte in attracting and/or stimulating the proliferation of mesenchymal cells1–5. We demonstrate here that activated human blood monocytes, but not resting monocytes, release a mediator that attracts smooth muscle cells and cooperates with other mediators to stimulate fibroblast proliferation. This mediator is very similar to platelet-derived growth factor (PDGF): its Chromatographic properties and chemical stability are similar to those of PDGF6,7, it competes with125I-PDGF for binding to fibroblasts and it immunoprecipitates with anti-PDGF antibodies. In parallel, stimulated monocytes, but not resting monocytes, express the c-sisproto-oncogene, a gene coding for one of the PDGF chains8,9, consistent with the concept that expression of the c-sisproto-oncogene may be involved in the ability of mononuclear phagocytes to modulate the accumulation of mesenchymal cells.