Molecular Characteristics and Pathogenicity of an Avian Leukosis Virus Isolated from Avian Neurofibrosarcoma
Molecular Characteristics and Pathogenicity of an Avian Leukosis Virus Isolated from Avian Neurofibrosarcoma
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DOI：
10.1637/9830-060711-reg.1
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发表时间：
2012-03-01
期刊：
影响因子：
1.4
通讯作者：
Umemura, Takashi
中科院分区：
文献类型：
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作者：
Ochi, Akihiro;Ochiai, Kenji;Umemura, Takashi
周围神经鞘瘤（PNST）是罕见的鸡，其病因仍有待阐明。本研究对日本本地鸡（Gallus gallus acuticus）自然发生的PNST进行了病理学检查，并对从肿瘤中分离的禽白血病病毒（ALV）株进行了分子生物学分析。鸡的颈部有一个坚实的皮下肿块。与相邻脊髓（C9-14）相连的肿块在显微镜下由梭形细胞组成的高度细胞组织组成，梭形细胞排列成交错的束、流和栅栏状，具有Verocay小体，细胞较少，胶原丰富。免疫组化，肿瘤细胞分为两种类型：神经束膜细胞阳性波形蛋白，葡萄糖转运蛋白1（GLUT 1），和claudin 1;和雪旺细胞阳性波形蛋白，偶尔阳性S-100 α/β，但GLUT 1阴性。根据这些发现，诊断为神经纤维肉瘤。从该肿瘤中分离到一株ALV（CTS_5371），经核苷酸序列测定和系统发育分析，该病毒为一株重组病毒。另外，实验感染显示CTS_5371诱导Schwnm细胞和神经束膜细胞增殖。这些结果表明，该ALV株具有诱导鸡PNST的能力。
Peripheral nerve sheath tumors (PNSTs) are rare in chickens and their etiology remains to be elucidated. In this study, a naturally occurring PNST in a Japanese native fowl (Gallus gallus domesticus) was pathologically examined and the strain of avian leukosis virus (ALV) isolated from the neoplasm was characterized by molecular biological analysis. The fowl presented with a firm subcutaneous mass in the neck. The mass, connected to the adjacent spinal cord (C9-14), was microscopically composed of highly cellular tissue of spindle cells arranged in interlacing bundles, streams, and palisading patterns with Verocay bodies and less cellular tissue with abundant collagen. Immunohistochemically, neoplastic cells were divided into two types: perineurial cells positive for vimentin, glucose transporter 1 (GLUT1), and claudin1; and Schwann cells positive for vimentin, occasionally positive for S-100 alpha/beta but negative for GLUT1. Based on these findings, a diagnosis of neurofibrosarcoma was made. The complete nucleotide sequence of an ALV strain, CTS_5371, isolated from the neoplasm was determined and phylogenetic analysis indicated that the strain was a novel recombinant virus from avian leukosis/sarcoma viruses previously reported. Additionally, experimental infection revealed that CTS_5371 induced the proliferation of Schwnm cells and perineurial cells. These results suggest that this ALV strain has the ability to induce PNSTs in chickens.