Screening of phenylalanine ammonia lyase in plant tissues, and retention of activity during dehydration
Screening of phenylalanine ammonia lyase in plant tissues, and retention of activity during dehydration
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DOI：
10.1002/jsfa.3126
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发表时间：
2008-03-01
影响因子：
4.1
通讯作者：
Kermode, Allison
中科院分区：
文献类型：
--
作者：
Goldson, Andrea;Lam, Melanie;Kermode, Allison
背景技术背景：苯丙氨酸解氨酶（PAL），天然封装在植物细胞的口服治疗，可能会提供一个潜在的替代治疗高苯丙氨酸血症患者，包括苯丙酮尿症。结果：PAL酶活性在籽粒幼苗中最高，以7 d龄红春小麦（Triticum aestivum L.）幼苗小麦幼苗叶片和根/胚乳的PAL活性分别为11.90 ± 2.64和6.48 ± 1.59 μ mol·h ~（-1）·g ~（-1）干重。在小麦幼苗叶组织中检测到分子量为74、83和103 kDa的PAL相关多肽，而在根/胚乳组织中仅检测到分子量为74 kDa的PAL相关多肽。研究了脱水法浓缩小麦幼苗苯丙氨酸解氨酶的方法。冷冻干燥被发现保留最PAL活性（> 90%的回收率在干重的基础上）相比，空气干燥和真空微波干燥的叶和根/胚乳samples.CONCLUSION：这项研究导致了更好地了解PAL活性和稳定性在植物组织中，并提供了基础，开发一种天然植物制剂作为膳食补充剂治疗高苯丙氨酸血症。(c)2007化学工业协会。
BACKGROUND: Oral therapy with phenylalanine ammonia lyase (PAL), naturally encapsulated in plant cells, may provide a potential alternative treatment for hyperphenylalaninemic patients, including those with phenylketonuria. Therefore different sources of plant tissue were investigated for PAL activity.RESULTS: Enzyme activity was highest in grain seedlings, with maximal enzyme activity in 7-day-old red spring wheat (Triticum aestivum L.) seedlings. The PAL activities of leaves and roots/endosperm of wheat seedlings were 11.90 +/- 2.64 and 6.48 +/- 1.59 mu mol h(-1) g(-1) dry weight respectively. Three PAL-related polypeptides with molecular weights of 74, 83 and 103 kDa were identified in wheat seedling leaf tissues, while only the 74 kDa polypeptide was detected in root/endosperm tissues. Dehydration was investigated as a method of concentrating PAL in wheat seedlings. Freeze-drying was found to retain the most PAL activity (> 90% recovery on a dry weight basis) compared with air drying and vacuum microwave drying for both leaf and root/endosperm samples.CONCLUSION: This study has led to a better understanding of PAL activity and stability in plant tissues and provides the basis for developing a natural plant preparation as a dietary supplement for the treatment of hyperphenylalaninemia. (c) 2007 Society of Chemical Industry.