Evidence for random endonucleolytic cleavages between messages in decay of Escherichia coli trp mRNA.
Evidence for random endonucleolytic cleavages between messages in decay of Escherichia coli trp mRNA.
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大肠杆菌 trp mRNA 衰变过程中信息之间随机核酸内切裂解的证据。
DOI：
10.1016/0022-2836(80)90388-5
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发表时间：
1980
影响因子：
5.6
通讯作者：
Kennell,D
中科院分区：
文献类型：
--
作者：
Lim,LW;Kennell,D
本文通过对大肠杆菌多顺反子信使RNA衰变群体的大小分析，研究了大肠杆菌多顺反子信使RNA衰变的机理。通过仅测量包含五种信息中最接近的（EmRNA）分子的大小，直接表明裂解可能发生在顺反子间连接处，并且每个分子的裂解或衰变独立于任何其他位点，即以随机动力学发生。小于全长大小的多顺反子片段并不是由操纵子内的过早终止引起的。这些结果与早期关于半乳糖操纵子的结论一致，并表明多顺反子mRNA衰变的基本机制可能与仅在顺反子间连接处发生的内部裂解相同。
The mechanism of decay of polycistronictrpmessenger RNA inEscherichia coliwas studied by size analyses of decaying populations. By measuring the sizes of only those molecules that contained the most proximal (EmRNA) of the five messages it was shown directly that cleavages occur, probably at intercistronic junctions, and each one does so independently of cleavages or decay at any other sites, i.e. occurs with random kinetics. The polycistronic fragments of less than full-length size did not arise from premature terminations within the operon. These results agree with earlier conclusions for thelacandgaloperons and suggest that the basic mechanisms of decay of polycistronic mRNAs are probably the same with internal cleavages occurring only at the intercistronic junctions.