Proteolysis of ICAM-1 on Human Oral Epithelial Cells by Gingipains
Proteolysis of ICAM-1 on Human Oral Epithelial Cells by Gingipains
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DOI：
10.1177/154405910308201007
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发表时间：
2003-10
影响因子：
7.6
通讯作者：
H. Tada;Shunji Sugawara;Eiji Nemoto;Takahisa Imamura;J. Potempa;J. Travis;H. Shimauchi;Haruhiko T
中科院分区：
文献类型：
--
作者：
H. Tada;Shunji Sugawara;Eiji Nemoto;Takahisa Imamura;J. Potempa;J. Travis;H. Shimauchi;Haruhiko T
Cysteine proteinases (gingipains) from Porphyromonas gingivalis are considered key virulence factors of severe periodontitis and host immune evasion. Since expression of intercellular adhesion molecule-1 (ICAM-1) on gingival epithelium is indispensable in polymorphonuclear leukocyte (PMN) migration at the site of periodontitis, we examined the effects of gingipains on the expression of ICAM-1 on human oral epithelial cell lines (KB and HSC-2) by flow cytometry and Western blotting. We found that three purified forms of gingipains efficiently reduced ICAM-1 expression on the cells in a time- and dose-dependent manner. Gingipains reduced the expression on fixed cells and degraded the ICAM-1 in the cell membranes, indicating that the reduction resulted from direct proteolysis. They then disturbed the ICAM-1-dependent adhesion of PMNs to the cells. These results indicate that gingipains cleave ICAM-1 on oral epithelial cells, consequently disrupting PMN-oral epithelial cell interaction, and are involved in immune evasion by the bacterium in periodontal tissues.
