Cre-mediated recombination in the skin melanocyte lineage
Cre-mediated recombination in the skin melanocyte lineage
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DOI：
10.1002/gene.10197
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发表时间：
2003-06-01
期刊：
影响因子：
1.5
通讯作者：
Larue, L
中科院分区：
文献类型：
--
作者：
Delmas, V;Martinozzi, S;Larue, L
Cre重组酶的器官特异性表达允许分析特定组织或细胞类型中的基因功能。使用6.1 kb的启动子从小鼠酪氨酸酶基因，我们产生和表征两个行的转基因小鼠表达Cre重组酶在成黑素细胞。利用Cre-反应性报告小鼠品系，从胚胎第11.5天起在皮肤的成黑素细胞中检测到遗传重组。此外，在小鼠的皮肤和眼睛中检测到Cre表达。偶尔在脑和周围神经中检测到Cre转基因活性，但在其他组织中未检测到。当Tyr：：Cre小鼠与携带胰岛素样生长因子受体基因（Igf 1 r）纯合loxP条件突变的小鼠杂交时，确认了Cre-成黑素细胞特异性重组模式，未观察到异常表型。总之，Tyr：：Cre转基因小鼠提供了一个有价值的工具，以遵循细胞谱系，并检查基因功能的黑素细胞的发展和转化。(C)2003 Wiley-Liss，Inc.
Organ-specific expression of a Cre recombinase allows the analysis of gene function in a particular tissue or cell type. Using a 6.1 kb promoter from the mouse tyrosinase gene, we generated and characterized two lines of transgenic mice that express Cre recombinase in melanoblasts. Utilizing a Cre-responsive reporter mouse strain, genetic recombination was detected in the melanoblasts of the skin from embryonic day 11.5. In addition, Cre-expression was detected in the skin and eyes of mice. Cre transgene activity was occasionally detected in the brain and peripheral nerves but not in other tissues. When Tyr::Cre mice were crossed with mice carrying a homozygous loxP conditional mutation for the insulin-like growth factor receptor gene (Igf1r), Cre-melanoblast-specific recombination pattern was confirmed and no abnormal phenotype was observed. In conclusion, Tyr::Cre transgenic mice provide a valuable tool to follow the cell lineage and to examine gene function in melanocyte development and transformation. (C) 2003 Wiley-Liss, Inc.