Mesostriatal and mesolimbic projections of midbrain neurons immunoreactive for estrogen receptor beta or androgen receptors in rats
Mesostriatal and mesolimbic projections of midbrain neurons immunoreactive for estrogen receptor beta or androgen receptors in rats
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DOI：
10.1002/cne.20229
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发表时间：
2004-08-30
影响因子：
2.5
通讯作者：
Kritzer, MF
中科院分区：
文献类型：
--
作者：
Creutz, LM;Kritzer, MF
大鼠尾壳核、杏仁核和杏仁核的多巴胺（DA）输入对循环雌激素和雄激素敏感。这些系统的激素调节的一种机制可能是通过对同源细胞内雌激素和雄激素受体的作用。然而，尽管已知中脑DA神经元的特定亚群对雌激素受体β（ER β）或雄激素受体（AR）呈免疫阳性，但尚不清楚这些受体携带细胞的投射部位。为了解决这个问题，我们结合双标记免疫细胞化学逆行追踪，以确定前脑的预测ER β或AR免疫反应（IR）中脑神经元。具体地说，Fluoro-Gold和/或霍乱毒素被注射到尾壳核、杏仁核或杏仁核的离散亚区。由此产生的中脑标记的评价显示，ER β-IR神经元发送侧支投射主要向腹侧尾状核壳核和杏仁核，但不向背侧尾状核或背侧杏仁核。与此相反，AR-IR神经元投射到杏仁核或延髓核，但不向尾壳核。这些前脑预测的组织与大鼠中纹状体和中脑边缘DA系统的一些已知的激素敏感性一致，并提供了一个解剖模型，该模型预测了雄激素和雌激素对纹状体和中脑边缘DA系统的单独影响。(C)2004威利-利斯公司
The dopamine (DA) inputs to the caudate putamen, the nucleus accumbens, and the amygdala in rats are sensitive to circulating estrogens and androgens. One mechanism for the hormone modulation of these systems may be via actions at cognate intracellular estrogen and androgen receptors. However, although it is known that specific subsets of midbrain DA neurons are immunopositive for estrogen receptor beta (ERbeta) or androgen receptors (ARs), it is not known where these receptor-bearing cells project. To address this issue, we combined double-label immunocytochemistry with retrograde tract tracing to identify the forebrain projections of ERbeta- or AR-immunoreactive (IR) midbrain neurons. Specifically, Fluoro-Gold and/or cholera toxin were injected into discrete subregions of the caudate-putamen, the nucleus accumbens, or the amygdala. Evaluations of the resultant midbrain labeling revealed that ERbeta-IR neurons sent collateral projections mainly to both the ventral caudate-putamen and the amygdala, but not to the dorsal caudate or nucleus accumbens. In contrast, AR-IR neurons projected either to the amygdala or the nucleus accumbens but not to the caudate-putamen. The organization of these forebrain projections concurs with some of the known hormone sensitivities of mesostriatal and mesolimbic DA systems in rats and provides an anatomical model that predicts separate influences for androgens and estrogens over mesostriatal and mesolimbic DA systems. (C) 2004 Wiley-Liss, Inc.