DOUBLE MINUTE CHROMOSOMES CAN BE PRODUCED FROM PRECURSORS DERIVED FROM A CHROMOSOMAL DELETION
DOUBLE MINUTE CHROMOSOMES CAN BE PRODUCED FROM PRECURSORS DERIVED FROM A CHROMOSOMAL DELETION
复制标题
DOI：
10.1128/mcb.8.4.1525
复制
发表时间：
1988-04-01
影响因子：
5.3
通讯作者：
WAHL, GM
中科院分区：
文献类型：
--
作者：
CARROLL, SM;DEROSE, ML;WAHL, GM
最近的实验表明，基因扩增可以介导的亚微观，自主复制，环状染色体外分子。我们将这些分子称为附加体（S.卡罗尔，P. L. DeRose，J. F.埃默里，J.L. Meinkoth，E. Nakkim，M. Subler，D. D. Von霍夫，G. M.沃尔，莫尔。Cell. 7：1740-1750，1987）。本文报道的实验探索了附加体的形成方式及其随时间在细胞中的命运。数据显示，在我们的系统中，附加体最初是250个染色体，但逐渐扩大，直到它们成为双微染色体。此外，我们发现游离体或双微体染色体可以整合到染色体中。我们的研究结果还表明，附加体可以通过从染色体上删除相应的序列产生。
Recent experiments have shown that gene amplication can be mediated by submicroscopic, autonomously replicating, circular extrachromosomal molecules. We refer to those molecules as episomes (S. Carroll, P. Gaudray, M. L. DeRose, J. F. Emery, J. L. Meinkoth, E. Nakkim, M. Subler, D. D. Von Hoff, and G. M. Wahl, Mol. Cell. Biol. 7:1740-1750, 1987). The experiments reported in this paper explore the way episomes are formed and their fate in the cell over time. The data reveal that in our system the episomes are initially 250 kilobases, but gradually enlarge until they become double minute chromosomes. In addition, we show that episomes or double minute chromosomes can integrate into chromosomes. Our results also suggest that episomes can be produced by deletion of the corresponding sequences from the chromosome.