maelstrom is required for an early step in the establishment of Drosophila oocyte polarity: posterior localization of grk mRNA.
maelstrom is required for an early step in the establishment of Drosophila oocyte polarity: posterior localization of grk mRNA.
复制标题
DOI：
10.1242/dev.124.22.4661
复制
发表时间：
1997-11
期刊：
影响因子：
4.6
通讯作者：
N. Clegg;D. Frost;Michele Keller Larkin;L. Subrahmanyan;Z. Bryant;H. Ruohola-Baker
中科院分区：
文献类型：
--
作者：
N. Clegg;D. Frost;Michele Keller Larkin;L. Subrahmanyan;Z. Bryant;H. Ruohola-Baker
我们描述了一种突变体，大漩涡，破坏了一个以前未观察到的步骤，mRNA定位在早期卵母细胞，不同的保育细胞卵母细胞RNA运输。大漩涡中的突变干扰了Gurken（果蝇Egf受体的配体）、Oskar和Bicoid的mRNA在发育中（3-6期）卵母细胞后部的定位。大漩涡突变体显示在Gurken功能丧失突变体中检测到的表型：后卵泡细胞具有前细胞命运，bicoid mRNA定位于第8阶段卵母细胞的两极和蛋壳的腹侧化。这些数据是一致的建议，早期后方本地化的gurken mRNA是必不可少的激活后卵泡细胞中的EGF受体通路。因此，3-6期卵母细胞中mRNA的后部定位可能是建立卵母细胞极性的最早已知步骤之一。大漩涡基因编码一种新的蛋白质，该蛋白质在滋养细胞和卵母细胞的细胞质中具有点状分布，直到卵子发生的第7阶段蛋白质消失。
We describe a mutant, maelstrom, that disrupts a previously unobserved step in mRNA localization within the early oocyte, distinct from nurse-cell-to-oocyte RNA transport. Mutations in maelstrom disturb the localization of mRNAs for Gurken (a ligand for the Drosophila Egf receptor), Oskar and Bicoid at the posterior of the developing (stage 3-6) oocyte. maelstrom mutants display phenotypes detected in gurken loss-of-function mutants: posterior follicle cells with anterior cell fates, bicoid mRNA localization at both poles of the stage 8 oocyte and ventralization of the eggshell. These data are consistent with the suggestion that early posterior localization of gurken mRNA is essential for activation of the Egf receptor pathway in posterior follicle cells. Posterior localization of mRNA in stage 3-6 oocytes could therefore be one of the earliest known steps in the establishment of oocyte polarity. The maelstrom gene encodes a novel protein that has a punctate distribution in the cytoplasm of the nurse cells and the oocyte until the protein disappears in stage 7 of oogenesis.