Zika virus evolution and spread in the Americas
Zika virus evolution and spread in the Americas
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DOI：
10.1038/nature22402
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发表时间：
2017-06-15
期刊：
影响因子：
64.8
通讯作者：
Sabeti, Pardis C.
中科院分区：
文献类型：
--
作者：
Metsky, Hayden C.;Matranga, Christian B.;Sabeti, Pardis C.
虽然最近寨卡病毒（ZIKV）在美洲的流行及其与出生缺陷的联系引起了极大的关注（1，2），但ZIKV疾病流行病学和ZIKV进化仍有许多未知之处，部分原因是缺乏基因组数据。在这里，我们通过使用多种测序方法从来自10个国家和地区的临床和蚊子样本中生成110个ZIKV基因组来解决这一知识差距，大大扩展了此次疫情中观察到的病毒遗传多样性。我们分析了引入不同地理区域的时间和模式;我们的系统发育证据表明，巴西的疫情迅速扩大，并将疫情菌株多次引入波多黎各、洪都拉斯、哥伦比亚、其他加勒比岛屿和美国大陆。我们发现ZIKV在多个地区传播了数月而未被发现，这突出了监测病毒感染的重要性。我们鉴定了对ZIKV生物学和发病机制具有可能功能意义的突变，以及可能与诊断测试的有效性相关的突变。
Although the recent Zika virus (ZIKV) epidemic in the Americas and its link to birth defects have attracted a great deal of attention(1,2), much remains unknown about ZIKV disease epidemiology and ZIKV evolution, in part owing to a lack of genomic data. Here we address this gap in knowledge by using multiple sequencing approaches to generate 110 ZIKV genomes from clinical and mosquito samples from 10 countries and territories, greatly expanding the observed viral genetic diversity from this outbreak. We analysed the timing and patterns of introductions into distinct geographic regions; our phylogenetic evidence suggests rapid expansion of the outbreak in Brazil and multiple introductions of outbreak strains into Puerto Rico, Honduras, Colombia, other Caribbean islands, and the continental United States. We find that ZIKV circulated undetected in multiple regions for many months before the first locally transmitted cases were confirmed, highlighting the importance of surveillance of viral infections. We identify mutations with possible functional implications for ZIKV biology and pathogenesis, as well as those that might be relevant to the effectiveness of diagnostic tests.