The synthesis of n-caproate from lactate: a new efficient process for medium-chain carboxylates production.
The synthesis of n-caproate from lactate: a new efficient process for medium-chain carboxylates production.
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从乳酸合成正己酸酯：一种高效的中链羧酸盐生产新工艺
DOI：
10.1038/srep14360
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发表时间：
2015-09-25
影响因子：
4.6
通讯作者：
Bo T
中科院分区：
文献类型：
--
作者：
Zhu X;Tao Y;Liang C;Li X;Wei N;Zhang W;Zhou Y;Yang Y;Bo T
从用于生产中国浓香型白酒（CSFL）的发酵池中获得了代谢乳酸而不是乙醇以生产正己酸的独特微生物组。在没有在线提取的分批试验中，微生物组能够以高达23.41 g/L的浓度以2.97 g/L/d的最大速率生产正己酸。与以前的工作相比，使用乙醇作为电子供体，正己酸根浓度增加了82.89%。高通量测序分析显示，微生物组由梭菌属簇IV主导，其占总读数的79.07%。提出了一种新的正己酸生产工艺，乳酸氧化耦合链延长，这揭示了新的见解，充分研究乳酸转化和碳链延长。此外，这些发现表明了一种新的正己酸酯在CSFL中的合成机理。我们相信，这种高效的工艺将为废物回收的创新以及正己酸生物合成提供一个有前途的机会。
A unique microbiome that metabolizes lactate rather than ethanol for n-caproate production was obtained from a fermentation pit used for the production of Chinese strong-flavour liquor (CSFL). The microbiome was able to produce n-caproate at concentrations as high as 23.41 g/L at a maximum rate of 2.97 g/L/d in batch trials without in-line extraction. Compared with previous work using ethanol as the electron donor, the n-caproate concentration increased by 82.89%. High-throughput sequencing analysis showed that the microbiome was dominated by a Clostridium cluster IV, which accounted for 79.07% of total reads. A new process for n-caproate production was proposed, lactate oxidation coupled to chain elongation, which revealed new insight into the well-studied lactate conversion and carbon chain elongation. In addition, these findings indicated a new synthesis mechanism of n-caproate in CSFL. We believe that this efficient process will provide a promising opportunity for the innovation of waste recovery as well as for n-caproate biosynthesis.