FORMATION OF PATTERN IN REGENERATING TISSUE PIECES OF HYDRA-ATTENUATA .1. HEAD-BODY PROPORTION REGULATION
FORMATION OF PATTERN IN REGENERATING TISSUE PIECES OF HYDRA-ATTENUATA .1. HEAD-BODY PROPORTION REGULATION
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DOI：
10.1016/0012-1606(80)90348-6
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发表时间：
1980-01-01
影响因子：
2.7
通讯作者：
BODE, HR
中科院分区：
文献类型：
--
作者：
BODE, PM;BODE, HR
定量测量了几乎均匀的水螅细胞片发育成完整动物的精确度。从成年水螅的体柱上切下不同尺寸的组织块并使其再生。对再生动物的头部（下体加触手环）、头部尝试（身体触手）和基底盘的数量进行了分析。为了确定头部和身体是否按正常比例改造，测量了头部和身体上皮细胞的平均数量。大小为成人 1/2 到 1/20 的组织碎片形成了头部，这些头部是再生者的恒定部分。在 10 倍的尺寸范围内，比例机制正在运行，可以非常精确地将组织分为头部区域和身体区域，但似乎达到了该范围的极限。再生体并非都是具有 1 个下口和 1 个基底盘的正常微型模型。当切片的宽长比增加到超过完整体柱的周长比时，头部和身体触手中额外的下口以及身体中额外的基盘的发生率急剧上升。提出了一种基于 Gierer-Meinhardt 模式形成模型的比例机制来解释结果。
The precision with which an almost uniform sheet of hydra cells develop into a complete animal was measured quantitatively. Pieces of tissue of varying dimensions were cut from the body column of an adult hydra and allowed to regenerate. The regenerated animals were assayed for number of heads (hypostomes plus tentacle rings), head attempts (body tentacles) and basal discs. To ascertain whether the head and body were reformed in normal proportions, the average number of epithelial cells in the heads and bodies was measured. Pieces of tissue, from 1/2 to 1/20 an adult in size, formed heads that were a constant fraction of the regenerate. Over a 10-fold size range, a proportioning mechanism was operating to divide the tissue into head area and body area quite precisely, but appeared to reach limits at the extremes of the range. The regenerates were not all normal miniatures with 1 hypostome and 1 basal disc. As the width-length ratio of the cut piece was increased beyond the circumference-length ratio of the intact body column, the incidence of extra hypostomes in the head and body tentacles, and of extra basal discs in the body, rose dramatically. A proportioning mechanism based on the Gierer-Meinhardt model for pattern formation is presented to explain the results.