Genetic dissection of the Leishmania paraflagellar rod, a unique flagellar cytoskeleton structure.
Genetic dissection of the Leishmania paraflagellar rod, a unique flagellar cytoskeleton structure.
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利什曼原虫副鞭毛杆的遗传解剖，这是一种独特的鞭毛细胞骨架结构。
DOI：
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发表时间：
1999
影响因子：
4
通讯作者：
Jonathan H. LeBowitz
中科院分区：
文献类型：
--
作者：
John A. Maga;Trevor Sherwin;S. Francis;Keith Gull;Jonathan H. LeBowitz
鞭毛旁杆（paraflagellar rod，PFR）是锥虫鞭毛中位于轴丝旁边的独特的细胞骨架细丝网络。虽然对墨西哥利什曼原虫PFR蛋白的两个主要组分PFR 1和PFR 2如何组装成这种复杂的结构知之甚少，但先前对PFR 2无效突变体的分析表明，PFR对适当的细胞运动性至关重要。现在通过比较PFR 2无效突变体与新的PFR 1无效突变体和PFR 1/PFR 2双无效突变体寄生虫来检查PFR 1和PFR 2的结构作用。PFR 1和PFR 2都是PFR形成和细胞运动所必需的。当消除一个PFR基因阻止组装的天然PFR结构，其他PFR蛋白积累在远端鞭毛尖端。比较每个突变体中剩余的PFR亚结构发现：（1）将PFR连接到轴丝的纤维不包含PFR 1或PFR 2，并且在PFR不存在的情况下组装。(2)在没有PFR 2的情况下，PFR 1被合成并运输到鞭毛，在那里它与轴丝附着纤维形成稳定的结合。(3)在没有PFR 1的情况下，PFR 2被合成并运输到鞭毛，尽管没有发现它与轴丝附着纤维相关。(4)PFR 1和PFR 2位于PFR的整个亚结构域。这些数据表明，虽然PFR丝包含PFR 1和PFR 2，PFR是通过PFR 1与轴丝附着纤维的相互作用附着到轴丝上的。
The paraflagellar rod (PFR) is a unique network of cytoskeletal filaments that lies alongside the axoneme in the flagella of most trypanosomatids. While little is known about how two major Leishmania mexicana PFR protein components, PFR1 and PFR2, assemble into this complex structure, previous analysis of PFR2 null mutants demonstrated that the PFR is essential for proper cell motility. The structural roles of PFR1 and PFR2 are now examined through comparison of PFR2 null mutants with new PFR1 null mutant and PFR1/PFR2 double null mutant parasites. Both PFR1 and PFR2 were essential for PFR formation and cell motility. When elimination of one PFR gene prevented assembly of a native PFR structure, the other PFR protein accumulated at the distal flagellar tip. Comparison of PFR substructures remaining in each mutant revealed that: (1) fibers that attach the PFR to the axoneme did not contain PFR1 or PFR2, and assemble in the absence of a PFR. (2) PFR1 was synthesized and transported to the flagella in the absence of PFR2, where it formed a stable association with the axoneme attachment fibers. (3) PFR2 was synthesized and transported to the flagella in the absence of PFR1, though it was not found associated with the axoneme attachment fibers. (4) PFR1 and PFR2 were located throughout the subdomains of the PFR. These data suggest that while PFR filaments contain both PFR1 and PFR2, the PFR is attached to the axoneme by interaction of PFR1 with the axoneme attachment fibers.
布氏锥虫副鞭毛杆的新型重复蛋白的分子特征。
影响因子：
1.5
作者：
Woodward,R;Carden,MJ;Gull,K
通讯作者：
Gull,K
克氏锥虫的鞭毛旁杆中存在两种不同的主要蛋白质成分 PAR 1 和 PAR 2 的证据。
DOI：
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发表时间：
1992
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Beard,CA;Saborio,JL;Tewari,D;Krieglstein,KG;Henschen,AH;Manning,JE
通讯作者：
Manning,JE
DOI：
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发表时间：
1989
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Saborio,JL;ManuelHernandez,J;Narayanswami,S;Wrightsman,R;Palmer,E;Manning,J
通讯作者：
Manning,J