Affinity Separations in Magnetically Stabilized Fluidized Beds: Synthesis and Performance of Packing Materials
Affinity Separations in Magnetically Stabilized Fluidized Beds: Synthesis and Performance of Packing Materials
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DOI：
10.1080/01496398708068603
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发表时间：
1987-11
影响因子：
2.8
通讯作者：
C. Lochmüller;L. Wigman
中科院分区：
文献类型：
--
作者：
C. Lochmüller;L. Wigman
摘要研制了一种磁稳定流化床分离器，用于测试薄膜状铁磁性亲和色谱填料的使用。使用绕线螺线管来产生磁场。铁磁包装材料由涂在聚苯乙烯珠上的含磁铁矿的聚氨酯凝胶组成。凝胶含有游离羧基。这些是与大豆胰蛋白酶抑制剂和用于胰蛋白酶纯化的材料偶联的马来酰亚胺。窄带亲和色谱在填充床、流化床和磁稳定流化床分离器中进行。对每种类型的分离器的压降、容量、稀释度和峰不对称性进行了评价。这三种类型提供了相当的效率，但流化分离器表现出低得多的压降。正如所预期的，流化床分离器对于亲和色谱法表现良好，但对于尺寸排阻色谱法表现较差。
Abstract A magnetically stabilized fluidized-bed separator designed to test the use of pellicular, ferromagnetic affinity chromatography packing materials has been developed. A wire wound solenoid was used to produce the magnetic field. The ferromagnetic packing material is comprised of a magnetite-containing, poly-urethane gel coated onto polystyrene beads. The gel contains free carboxyl groups. These were carbodiimide-coupled to soy-trypsin inhibitor and the material, used for trypsin purification. Narrow-band affinity chromatography was carried out in packed-bed, fluidized-bed and magnetically stabilized, fluidized-bed separators. Pressure drop, capacity, dilution, and peak asymmetry were evaluated for each type of separator. The three types provide comparable efficiency but the fluidized separators exhibit a much lower pressure drop. As might be expected, fluidized-bed separators perform well for affinity chromatography Oarge k') but poorly for size exclusion chromatography.