Reclassification of the Candida haemulonii Complex as Candida haemulonii (C. haemulonii Group I), C. duobushaemulonii sp. nov. (C. haemulonii Group II), and C. haemulonii var. vulnera var. nov.: Three Multiresistant Human Pathogenic Yeasts
Reclassification of the Candida haemulonii Complex as Candida haemulonii (C. haemulonii Group I), C. duobushaemulonii sp. nov. (C. haemulonii Group II), and C. haemulonii var. vulnera var. nov.: Three Multiresistant Human Pathogenic Yeasts
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DOI：
10.1128/jcm.02248-12
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发表时间：
2012-11-01
影响因子：
9.4
通讯作者：
Boekhout, T.
中科院分区：
文献类型：
--
作者：
Cendejas-Bueno, E.;Kolecka, A.;Boekhout, T.
该念珠菌属复合体目前被称为C。Haemulonii组I和II。这里我们描述C。haemulonii Ⅱ组为一新种，杜布沙木隆假丝酵母Candida duobushaemulonii sp. nov.，和C.黑穆隆原变种新变种C. Haemulonii I组的表型和分子生物学方法。这些分类群和C. Haemulonii（即，耳念珠菌和假海穆隆念珠菌）不能通过目前用于酵母菌鉴定的商业方法区分。4个分离物（C.黑穆隆原变种Escherichia）与其他C. haemulonii在核rRNA基因操纵子的内转录间隔区（ITS）序列中的差异。新种和新变种具有多重耐药性抗真菌特征，其中包括高MIC的阿替霉素B（几何平均MIC，1.18 mg/L，C.黑穆隆原变种2mg/L; duobushaemulonii sp. nov）和对唑类化合物的交叉抗性。这些物种的鉴定应基于分子方法，如ITS区序列分析和基质辅助激光解吸电离飞行时间质谱。
The Candida haemulonii species complex is currently known as C. haemulonii groups I and II. Here we describe C. haemulonii group II as a new species, Candida duobushaemulonii sp. nov., and C. haemulonii var. vulnera as new a variety of C. haemulonii group I using phenotypic and molecular methods. These taxa and other relatives of C. haemulonii (i.e., Candida auris and Candida pseudohaemulonii) cannot be differentiated by the commercial methods now used for yeast identification. Four isolates (C. haemulonii var. vulnera) differed from the other isolates of C. haemulonii in the sequence of the internal transcribed spacer (ITS) regions of the nuclear rRNA gene operon. The new species and the new variety have a multiresistant antifungal profile, which includes high MICs of amphotericin B (geometric mean MIC, 1.18 mg/liter for C. haemulonii var. vulnera and 2 mg/liter for C. duobushaemulonii sp. nov) and cross-resistance to azole compounds. Identification of these species should be based on molecular methods, such as sequence analysis of ITS regions and matrix-assisted laser desorption ionization-time of flight mass spectrometry.