The possibility of visualizing TGF-β1 expression in ApoE-/- mice atherosclerosis using MR targeted imaging.
The possibility of visualizing TGF-β1 expression in ApoE-/- mice atherosclerosis using MR targeted imaging.
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DOI：
10.1177/02841851231153989
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发表时间：
2023-02
期刊：
影响因子：
1.3
通讯作者：
M. Xia;Feng Wu;Yawen Yang;Wenye Lu;Mengxing Song;Zhanlong Ma
中科院分区：
文献类型：
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作者：
M. Xia;Feng Wu;Yawen Yang;Wenye Lu;Mengxing Song;Zhanlong Ma
背景内皮转化生长因子-β1信号是动脉粥样硬化相关血管炎症的主要驱动因素。靶向成像和抑制转化生长因子-β-1的表达可减轻动脉粥样硬化的管壁炎症，阻止动脉粥样硬化斑块的发展。目的探讨抗转化生长因子-β-1超小分子超顺磁性氧化铁探针作为7.0T磁共振成像检测载脂蛋白E-/-小鼠动脉粥样硬化中转化生长因子-β-1表达的可能性。材料与方法以70只高脂饮食的ApoE-/-小鼠为实验组，30只正常饮食的C57BL/6小鼠为对照组。H&E染色观察斑块形态，免疫组织化学染色检测肿瘤坏死因子和转化生长因子-β-1的表达和分布。另取40只小鼠分为靶向组和单纯组，分别注射抗转化生长因子-β1-USPIO探针和单纯USPIO。结果7.0TMRI显示靶组相对信号强度较单纯组明显降低(−为19.34±0.68%，−为5.61±0.57%；P<；0.05)。组织病理学分析显示转化生长因子-β1在动脉粥样硬化斑块形成过程中的表达从10周到28周。转化生长因子-β1在动脉粥样硬化中的表达与动脉粥样硬化斑块形成的病理过程有较好的相关性。结论抗转化生长因子-β1-USPIO可作为一种有用的分子成像工具，用于检测和监测动脉粥样硬化斑块中转化生长因子-β1的表达。
Background Endothelial TGF-β1 signaling is a primary driver of atherosclerosis-associated vascular inflammation. Targeted imaging and inhibition of the expression of TGF-β1 may reduce the atherosclerotic vessel wall inflammation and stop the progression of atherosclerotic plaque. Purpose To investigate the possibility of the anti-TGF-β1-ultrasmall superparamagnetic iron oxide (USPIO) specific probe as an imaging marker for the expression of TGF-β1 in ApoE–/– mice atherosclerosis detected with 7.0-T magnetic resonance imaging (MRI). Material and Methods Here, 70 ApoE–/– mice on a high-fat diet served as the experimental group and 30 C57BL/6 mice on a normal diet served as the control group. The morphology of plaques was viewed by H&E staining, and the expression and distribution of TNC and TGF-β1 were detected by immunohistochemical staining. Another 40 mice in the experimental group were classified into a targeted group, which was administrated an anti-TGF-β1-USPIO probe, and the pure group, which was injected with pure USPIO. Results The 7.0-T MRI showed that the relative signal intensity (rSI) changes of the targeted group decreased more than those of the pure group (−19.34 ± 0.68% vs. −5.61 ± 0.57%; P < 0.05). Histopathological analyses demonstrated expression of TGF-β1 in atherosclerotic plaque formation progression from 10 to 28 weeks. The MR images of the expression of TGF-β1 in atherosclerosis correlated well with the pathological progression of atherosclerotic plaque formation. Conclusions Anti-TGF-β1-USPIO could provide a useful molecular imaging tool for detecting and monitoring the expression of TGF-β1 in atherosclerotic plaques by MRI.