NATURAL KILLER CELLS IN MOUSE .1. CYTOTOXIC CELLS WITH SPECIFICITY FOR MOUSE MOLONEY LEUKEMIA-CELLS - SPECIFICITY AND DISTRIBUTION ACCORDING TO GENOTYPE
NATURAL KILLER CELLS IN MOUSE .1. CYTOTOXIC CELLS WITH SPECIFICITY FOR MOUSE MOLONEY LEUKEMIA-CELLS - SPECIFICITY AND DISTRIBUTION ACCORDING TO GENOTYPE
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DOI：
10.1002/eji.1830050208
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发表时间：
1975-01-01
影响因子：
5.4
通讯作者：
WIGZELL, H
中科院分区：
文献类型：
--
作者：
KIESSLING, R;KLEIN, E;WIGZELL, H
在年轻成年小鼠的脾脏中，存在对小鼠莫洛尼氏白血病细胞具有特异性的天然杀伤淋巴细胞。对同基因或异基因莫洛尼白血病细胞的裂解活性在相似程度上是直接的，但主要在体外培养的白血病细胞中表达。两种非莫洛尼起源的白血病是耐药的，肥大细胞瘤系P815也是如此。虽然不同品系小鼠的杀伤活性不同，但裂解的特异性与使用未标记的莫洛尼或其他肿瘤细胞作为细胞毒性试验中的抑制剂的竞争实验所示相同。发现竞争能力和对杀伤细胞裂解的敏感性高度正相关。细胞毒性试验的动力学分析显示，在1至4小时内快速诱导裂解，这与任何常规的体外诱导免疫应答相反。未发现可溶性因子在细胞溶解试验中发挥任何作用的证据。
In the spleens of young, adult mice there exist naturally occurring killer lymphocytes with specificity for mouse Moloney leukemia cells. The lytic activity was directed against syngeneic or allogeneic Moloney leukemia cells to a similar extent, but was primarily expressed when tested againstin vitrogrown leukemia cells. Two leukemias of non‐Moloney origin were resistant and so was the mastocytoma line P815. Although killer activity varied between different strains of mice, the specificity of lysis was the same as indicated by competition experiments using unlabeled Moloney or other tumor cells as inhibitors in the cytotoxic assays. Capacity to compete and sensitivy to lysis by the killer cells were found to be highly positively correlated. Analysis of the kinetics of the cytotoxic assay revealed a rapid induction of lysis within one to four hours, arguing against any conventionalin vitroinduction of immune response. No evidence was found of soluble factors playing any role in the cytolytic assay.