Role of ATP in the intramitochondrial sorting of cytochrome c1 and the adenine nucleotide translocator.
Role of ATP in the intramitochondrial sorting of cytochrome c1 and the adenine nucleotide translocator.
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ATP 在细胞色素 c1 和腺嘌呤核苷酸易位子线粒体内分选中的作用。
DOI：
10.1002/j.1460-2075.1992.tb05584.x
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
Glick,BS
中科院分区：
文献类型：
--
作者：
Wachter,C;Schatz,G;Glick,BS
前体蛋白通过线粒体内膜的输入需要基质中的ATP。然而，一些前体仍然可以在ATP耗尽的线粒体中穿过外膜。在这里，我们表明腺嘌呤核苷酸转运体在基质耗尽ATP后正常进入内膜。这一结果支持了先前的建议，即转运体插入内膜而不通过基质。基质ATP的耗尽对细胞色素c1的输入和成熟也没有可检测到的影响，细胞色素c1是针对膜间空间的。因此，细胞色素c1在其输入途径中可能没有完全穿过内膜。
Import of precursor proteins across the mitochondrial inner membrane requires ATP in the matrix. However, some precursors can still cross the outer membrane in ATP‐depleted mitochondria. Here we show that the adenine nucleotide translocator is imported normally into the inner membrane after the matrix has been depleted of ATP. This result supports the earlier suggestion that the translocator inserts into the inner membrane without passing through the matrix. Depletion of matrix ATP also has no detectable effect on the import and maturation of cytochrome c1, which is targeted to the intermembrane space. It thus seems probable that cytochrome c1 does not completely cross the inner membrane during its import pathway.