Technological advances in bifidobacterial molecular genetics: application to functional genomics and medical treatments.
Technological advances in bifidobacterial molecular genetics: application to functional genomics and medical treatments.
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双歧杆菌分子遗传学的技术进步：应用于功能基因组学和医学治疗。
DOI：
10.12938/bmfh.31.15
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
Yokota A
中科院分区：
文献类型：
--
作者：
Fukiya S;Hirayama Y;Sakanaka M;Kano Y;Yokota A
双歧杆菌是众所周知的有益的肠道细菌，在人类中发挥健康促进作用。除了生理学和免疫学研究外，分子遗传学技术已经发展，最近开始应用于阐明宿主-双歧杆菌相互作用的分子基础。这些技术包括转化技术和能够实现异源基因表达的大肠杆菌-双歧杆菌穿梭载体。在这种情况下，为了提高转化效率，最近开发了一种质粒人工修饰方法，该方法保护引入的质粒不受宿主双歧杆菌中的限制性系统的影响。另一方面，对功能基因组学至关重要的靶向基因失活系统似乎远不能实际应用于双歧杆菌。然而，这项技术最近取得了显着的进展，使生物多样性细菌的功能基因组学。这些分子遗传技术与基于组学的分析的综合使用肯定会促进对双歧杆菌有益作用的分子基础的表征。重组双歧杆菌在医学治疗中的应用也取得了进展。
Bifidobacteria are well known as beneficial intestinal bacteria that exert health-promoting effects in humans. In addition to physiological and immunological investigations, molecular genetic technologies have been developed and have recently started to be applied to clarify the molecular bases of host-Bifidobacterium interactions. These technologies include transformation technologies and Escherichia coli-Bifidobacterium shuttle vectors that enable heterologous gene expression. In this context, a plasmid artificial modification method that protects the introduced plasmid from the restriction system in host bifidobacteria has recently been developed to increase transformation efficiency. On the other hand, targeted gene inactivation systems, which are vital for functional genomics, seemed far from being practically applicable in bifidobacteria. However, remarkable progress in this technology has recently been achieved, enabling functional genomics in bifidobacteria. Integrated use of these molecular genetic technologies with omics-based analyses will surely boost characterization of the molecular basis underlying beneficial effects of bifidobacteria. Applications of recombinant bifidobacteria to medical treatments have also progressed.