Alternative splicing regulates the transcriptional activity of Drosophila heat shock transcription factor in response to heat/cold stress
Alternative splicing regulates the transcriptional activity of Drosophila heat shock transcription factor in response to heat/cold stress
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DOI：
10.1016/j.febslet.2005.05.074
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发表时间：
2005-07-04
期刊：
影响因子：
3.5
通讯作者：
Toda, T
中科院分区：
文献类型：
--
作者：
Fujikake, N;Nagai, Y;Toda, T
热休克转录因子1 （HSF1）介导热休克蛋白的诱导，以应对各种类型的应激。虽然HSF1的活性受其翻译后修饰的调节，但mRNA表达的改变也被认为是可能的。我们在此鉴定了果蝇HSF (dHSF) mRNA的三个新的选择性剪接异构体，分别命名为dHSFb、dHSFc和dHSFd。我们发现dHSFb的比例在热暴露时增加，而dHSFd的比例在冷暴露时增加。dHSFc和dHSFd亚型的转录活性高于其他亚型。我们的研究结果表明，选择性剪接调节dHSF的转录活性。(c) 2005年欧洲生化学会联合会。Elsevier B.V.版权所有。
Heat shock transcription factor 1 (HSF1) mediates the induction of heat shock proteins in response to various types of stress. Although HSF1 activity is regulated by its post-translational modifications, alterations in mRNA expression have also been suggested. We here identified three new alternatively spliced isoforms of Drosophila HSF (dHSF) mRNA, named dHSFb, dHSFc, and dHSFd. We found that the ratio of dHSFb increases upon heat exposure, while that of dHSFd increases upon cold exposure. The dHSFc and dHSFd isoforms showed greater transcriptional activity than the other isoforms. Our findings suggest that alternative splicing regulates the transcriptional activity of dHSF. (c) 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.